当前位置: 首页 > 文章 > 也门铁的组织培养技术研究 亚热带植物科学 2015,44 (1) 23-28
Position: Home > Articles > Tissue Culture Technique of Dracaena fragrans Subtropical Plant Science 2015,44 (1) 23-28

也门铁的组织培养技术研究

作  者:
齐安民;王家琼;李楠;贺涛;李薇;崔大方
单  位:
广州南沙明珠湾区开发有限公司;华南农业大学公共基础课实验教学中心;华南农业大学林学院
关键词:
也门铁;组织培养;褐化
摘  要:
以4品种也门铁的茎段为外植体进行组织培养技术研究。结果表明,控制普通也门铁茎段褐化效果最理想的培养基为MS+0.25 g·L-1 VC+0.50 g·L-1 Na2S2O3;诱导也门铁不定芽萌动的最佳培养基为MS+3.0 mg·L-16-BA+0.2 mg·L-1 NAA+0.1 mg·L-1 KT;诱导也门铁不定芽增殖的最佳培养基,因品种不同而异,普通也门铁和金心也门铁为MS+2.0 mg·L-1 6-BA+1.0 mg·L-1 NAA+0.1 mg·L-1 GA3,扭纹铁和金心扭纹铁为MS+1.0 mg·L-16-BA+0.5 mg·L-1 NAA+0.1 mg·L-1 GA3;也门铁壮苗的最佳培养基为MS+0.05 mg·L-1 6-BA+0.05 mg·L-1 NAA+l g·L-1AC;也门铁生根最优培养基为1/2MS+0.5 mg·L-1 IBA。
译  名:
Tissue Culture Technique of Dracaena fragrans
作  者:
QI An-min;WANG Jia-qiong;LI Nan;HE Tao;LI Wei;CUI Da-fang;College of Forestry, South China Agricultural University;Yunyang Fruit Industry Development Council;Fengjie Forestry Bureau;Center of Experimental Teaching for common Basic Courses, South China Agricultural University;Nansha Pearl Bay Development Co. Ltd.;
关键词:
Dracaena fragrans;;tissue culture;;callus browning
摘  要:
The tissue culture techniques of four cultivars of Dracaena fragrans used stem section as the explant were analyzed. A series of important conclusions can be made: 1) The optimal medium for controlling tissue browning of D. fragrans: MS + 0.25 g·L-1 VC + 0.50 g·L-1 Na2S2O3; 2) The optimal medium for inducing the D. fragrans stem stirring: MS+ 3.0 mg·L-1 6-BA + 0.2 mg·L-1 NAA + 0.1 mg·L-1KT; 3) The optimal proliferation medium of D. fragrans, depends on the cultivars of D. fragrans. The optimal medium for D. fragrans and D. fragrans ‘Massangeana': MS + 2.0 m g·L-1 6-BA + 1.0 mg·L-1NAA + 0.1 mg·L-1 GA3; The optimal medium for D. fragrans ‘Compacta' and D. fragrans ‘Janet-craig': MS + 1.0 m g·L-1 6-BA + 0.5 mg·L-1 NAA + 0.1 mg·L-1 GA3; 4) Under the condition of MS + 0.05 mg·L-16-BA + 0.05 mg·L-1 NAA + l g·L-1 AC was the most efficient the Dracaena fragrans seedling medium; 5) 1/2MS + 0.5 mg·L-1 IBA was the most efficient rooting medium.

相似文章

计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊