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当前位置: 首页 > 文章 > 17α-甲基睾酮对斑马鱼肝脏vtg基因mRNA表达的影响 畜牧与饲料科学 2016,37 (5) 9-11
Position: Home > Articles > Effect of 17α-methyltestosterone(MT) on m RNA Expression of vtg Gene in Liver of Brachydanio rerio Animal Husbandry and Feed Science 2016,37 (5) 9-11

17α-甲基睾酮对斑马鱼肝脏vtg基因mRNA表达的影响

作  者：
刘少贞;吕文琪;吕小也
单  位：
山西农业大学动物科技学院
关键词：
17α-甲基睾酮;斑马鱼;肝脏;vtg
摘  要：
为评价17α-甲基睾酮(17α-methyltestosterone,MT)对斑马鱼肝脏vtg基因m RNA表达的影响,利用MT处理斑马鱼雌、雄鱼7 d,采用实时定量PCR(q RT-PCR)检测雌、雄鱼肝脏中vtg基因m RNA的表达量。结果显示,MT处理雌鱼7 d,25 ng/L处理组肝脏vtg基因的表达量显著降低(P<0.05),50 ng/L和100 ng/L的MT处理组肝脏vtg基因m RNA表达量极显著降低(P<0.01);MT处理雄鱼7 d,25 ng/L处理组肝脏vtg基因m RNA表达量与对照组相比差异不显著(P>0.05),50 ng/L的MT处理组肝脏vtg基因m RNA表达量显著升高(P<0.05),100 ng/L的MT处理组肝脏vtg基因m RNA表达量极显著升高(P<0.01)。斑马鱼肝脏vtg基因对MT比较敏感,可以作为监测环境中MT的生物标志物。
译  名：
Effect of 17α-methyltestosterone(MT) on m RNA Expression of vtg Gene in Liver of Brachydanio rerio
作  者：
LIU Shao-zhen;L Wen-qi;L Xiao-ye;College of Animal Science and Veterinary Medicine,Shanxi Agricultural University;
关键词：
17α-methyltestosterone;;Brachydanio rerio;;liver;;vtg
摘  要：
This study aimed to evaluate the effect of 17α-methyltestosterone(MT) on m RNA expression of vtg gene in liver of Brachydanio rerio. The male and female Brachydanio rerio were treated with different concentrations of MT for 7 days, and q RTPCR assay were used to determine the m RNA expression of vtg gene in their livers. For female Brachydanio rerio, the m RNA expression of vtg gene in 25 ng/L MT treatment group was significantly reduced after 7 days(P<0.05), and that in 50 and 100 ng/L MT treatment group was extremely significantly reduced(P <0.01). For male Brachydanio rerio, there were no significant differences in the m RNA expression of vtg gene between 25 ng/L MT treatment group and control group after 7 days(P>0.05).However, the m RNA expression of vtg gene in 50 and 100 ng/L MT treatment groups was significantly and extremely significantly increased(P<0.05 and P<0.01), respectively. The results suggest that the vtg gene in liver of Brachydanio rerio is sensitive to MT,and it is potential to serve as a biomarker for MT monitoring in environment.

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