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Position: Home > Articles > Simultaneous Determination of 11 Flavonoids in Citrus Fruits by Ultra Performance Liquid Chromatography FOOD SCIENCE 2013,34 (4) 168-172

超高效液相色谱法同时测定柑橘中11种类黄酮物质

作  者：
冉玥;焦必宁;赵其阳;田玲;苏学素;曾朝波
单  位：
西南大学食品科学学院;农业部柑橘产品质量安全风险评估实验室;西南大学化学化工学院
关键词：
超高效液相色谱;柑橘;类黄酮;正交试验
摘  要：
通过正交试验优化样品前处理条件,建立超高效液相色谱快速检测柑橘中11种类黄酮物质的方法。以甲醇-乙酸溶液为流动相,梯度洗脱,8种黄烷酮和3种多甲氧基黄酮11min内完全基线分离(检测波长283nm和330nm)。方法检出限为0.005～0.02mg/kg(RSN=3),线性方程的相关系数均达到0.9993以上。果皮和果汁的回收率分别为94.6%～100.2%,94.5%～101.8%;相对标准偏差(n=6)分别为1.1%～3.7%,0.9%～3.9%。该方法快速、高效、低成本、环境友好、结果准确可靠,能满足柑橘中类黄酮化合物的检测要求。
译  名：
Simultaneous Determination of 11 Flavonoids in Citrus Fruits by Ultra Performance Liquid Chromatography
作  者：
RAN Yue1,2,JIAO Bi-ning1,2,ZHAO Qi-yang2,TIAN Ling2,SU Xue-su3,ZENG Chao-bo3(1.College of Food Science,Southwest University,Chongqing 400715,China;2.Laboratory of Risk Assessment for Citrus Quality and Safety,Ministry of Agriculture,Citrus Research Institute,Southwest University,Chongqing 400712,China; 3.College of Chemistry and Chemical Engineering,Southwest University,Chongqing 400716,China)
关键词：
ultra performance liquid chromatography(UPLC);citrus;flavonoid;orthogonal experiment
摘  要：
A rapid method for qualitative and quantitative analysis of 11 flavonoids in citrus was developed using ultra performance liquid chromatography(UPLC) coupled with photodiode array detector.Under the condition of methanol-.acetic acid water solution as mobile phase and gradient elution,8 flavanones and 3 polymethacrylate flavones were separated within 11 min.The detection wavelengths were 283 nm and 330 nm.The limits of detection were from 0.005 to 0.02 mg/kg(RSN = 3) and good linearity was observed with the correlation coefficient above 0.9993.The influencing factors for sample preparation were optimized by using orthogonal experiment design and the recoveries of compounds analyzed from peel and juice samples were over the range of 94.6%–100.2% with RSD between 1.1% and 3.7%,and within the range of 94.5%–101.8% with RSD between 0.9% and 3.9%,respectively.Compared with the previously reported methods,this method was more effective,environmentally friendly and reproducible.

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