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Position: Home > Articles > Surfactant Precipitation and Recovery of Protamine FOOD SCIENCE 2010,31 (13) 56-59

表面活性剂沉淀鱼精蛋白及复溶回收过程研究

作  者:
刘琳;付朝霞;刘俊果;杜立业;赵紫华
单  位:
河北科技大学科研处;河北科技大学生物科学与工程学院
关键词:
鱼精蛋白;表面活性剂;沉淀;纯化
摘  要:
采用两种阴离子型表面活性剂:十二烷基硫酸钠(SDS)和2-乙基己基琥珀酸酯磺酸钠(AOT)沉淀鱼精蛋白并用极性有机溶剂从沉淀中复溶回收鱼精蛋白。结果表明:SDS可以完全沉淀鱼精蛋白,而表面活性剂AOT最高只能沉淀75.02%的鱼精蛋白;极性有机溶剂的种类及辅助无机盐的添加量,对从鱼精蛋白-表面活性剂难溶复合物中回收鱼精蛋白有很大的影响;对于SDS-鱼精蛋白复合物,正丙醇为最佳回收剂,鱼精蛋白最高回收率可达83.33%;对于AOT-鱼精蛋白复合物,丙酮为最佳回收剂,鱼精蛋白最高回收率达到85.71%。回收后的鱼精蛋白抗菌活性同原始鱼精蛋白相比没有明显变化。
译  名:
Surfactant Precipitation and Recovery of Protamine
作  者:
LIU Lin1,2,FU Zhao-xia3,LIU Jun-guo1,2,*,DU Li-ye1,ZHAO Zi-hua1 (1. College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China; 2. R&D Center for Fermentation Engineering of Hebei Province, Shijiazhuang 050018, China ; 3. Department of Scientific Research, Hebei University of Science and Technology, Shijiazhuang 050018, China)
关键词:
protamine;surfactant;precipitation;purification
摘  要:
The precipitation and recovery of protamine was investigated using ionic surfactants as precipitating ligands and polar organic solvents as recovery agents. Ionic surfactant such as sodium dodecyl sulfate (SDS) exhibited a complete removal of protamine; however, sodium di-(2-ethylhexyl) sulfosuccinate (AOT) exhibited only 75.02% removal of protamine. In addition, an obvious effect of organic solvent and salt amount on the recovery of protamine from its precipitate. As for SDS-protamine precipitate, n-propanol was the optimal solvent with protamine recovery rate of more than 83.33%. As for AOT-protamine precipitate, acetone was the optimal solvent with protamine recovery rate of more than 85.71%. Compared with original protamine, no significant change in antibacterial activity of recovered protamine was observed.

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