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当前位置: 首页 > 文章 > 纳滤技术浓缩纯化玉米肽及对其醒酒活性的影响 食品科学 2011,32 (8) 10-14
Position: Home > Articles > Nanofiltration for Concentration and Purification of Corn Peptides and Their Effects on Antialcoholism Activity FOOD SCIENCE 2011,32 (8) 10-14

纳滤技术浓缩纯化玉米肽及对其醒酒活性的影响

作  者：
于国才;何慧;靳桢;黄文浩;李江涛
单  位：
华中农业大学食品科学技术学院
关键词：
纳滤;玉米肽;醒酒;透析;脱盐;乙醇脱氢酶
摘  要：
探讨纳滤技术浓缩纯化玉米肽的过程中肽的醒酒活性的变化。本研究选用截留分子质量160D和360D的两种纳滤膜,对pH8.0玉米肽液(Mw<5kD)进行浓缩纯化,比较两种膜对其中主要成分的传质效果,并进一步探讨透析过滤过程对玉米肽醒酒活性的影响。结果表明,玉米肽液浓缩过程中,相对于360D纳滤膜,160D纳滤膜对肽类、氨基酸和Na+均具有较低的传质效率,但其对玉米肽的截留率却在88.80%以上,脱盐效果与360D膜相当,经3次透析后,脱盐率达94.6%,且160D纳滤膜在透析过程中很好地保持玉米肽对乙醇脱氢酶(ADH)的激活作用。结论:宜选用160D纳滤膜对玉米肽液进行浓缩纯化,透析过滤3次。
译  名：
Nanofiltration for Concentration and Purification of Corn Peptides and Their Effects on Antialcoholism Activity
作  者：
YU Guo-cai,HE Hui,JIN Zhen,HUANG Wen-hao,LI Jiang-tao(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)
关键词：
nanofiltration(NF);corn peptides;antialcoholism;dialysis;desalination;alcohol dehydrogenase(ADH)
摘  要：
Nanofiltration membranes(NFM)with molecular weight cut off(MWCO)160 D and 360 D were used to concentrate and purify corn protein hydrolysate of MW<5 kD at pH 8.0.The mass transfer efficiencies of peptides,amino acids and sodium ion(Na+)in concentration process and the changes in hydroxyl free radical scavenging activity and antialcoholism activity of corn peptides(CPs)in dialysis process were evaluated.The results showed that although 160 NFM exhibited lower transmission/ rejection rates of CPs,amino acids and Na+as compared to 360 D NFM,the rejection rate of CPs by 160 D NFM was above 88. 80%and it provided desalting effect similar to that of 360 D NFM and yielded a desalting efficiency of 94.6%after triple repeated dialysis.Moreover,the activating effect of corn peptides on alcohol dehydrogenase(ADH)was kept very well in 160 D NFM dialysis.In general,160 D NFM is suitable to be used for the concentration and purification of corn protein hydrolysate and the number of repeated dialysis should be no more than 3.

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