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Position: Home > Articles > 一株强致病力软腐病菌鉴定与室内药剂筛选 Plant Protection 2019 (4) 149-155

一株强致病力软腐病菌鉴定与室内药剂筛选

作  者：
李苗苗;伍翔;严准;吴中军;高春生;刘新宇;程毅
单  位：
河北北方学院植物保护系;重庆文理学院林学与生命科学学院;中国农业科学院麻类研究所南方经济作物研究中心;北京图为生物科技有限公司;中国农业科学院麻类研究所
关键词：
软腐病菌;看家基因;迪基氏菌属;MLSA;最小抑菌浓度
摘  要：
通过对从湘西武陵山区感病萝卜分离的一株软腐病菌进行致病力测定,结合萝卜软腐病菌的培养特性、菌体形态观察、生理生化测定及6个看家基因(gapA,icdA,pgi,rpoS,mdh,proA)的多位点序列分析(MLSA),结果表明,病原细菌Secpp 1600属革兰氏阴性菌,呈杆状,致病力强,可侵染分属8科13个属的多种经济作物;与迪基氏菌Dickeya fangzhongdai菌株高度同源.选用7种药剂通过抑菌圈法对Secpp 1600进行了抑菌效果初步测定,结果表明,农用链霉素、SPP3和SPP4可抑制病菌生长,药剂间抑菌率存在显著差异,其中,SPP4的直接抑菌效果最好,最小抑制浓度为900μg/mL.
作  者：
LI Miaomiao;WU Xiang;YAN Zhun;WU Zhongjun;GAO Chunsheng;LIU Xinyu;Cheng Yi;Institute of Bast Fibre Crops and Centre of Southern Economic Crops,Chinese Academy of Agricultural Sciences;College of Horticulture and Landscape Architecture,Southwest University;Beijing Tuwei Biotech Co.,Ltd.;College of Forestry and Life Sciences,Chongqing University of Arts and Sciences;Department of Plant Protection,Hebei North University;
单  位：
LI Miaomiao%WU Xiang%YAN Zhun%WU Zhongjun%GAO Chunsheng%LIU Xinyu%Cheng Yi%Institute of Bast Fibre Crops and Centre of Southern Economic Crops,Chinese Academy of Agricultural Sciences%College of Horticulture and Landscape Architecture,Southwest University%Beijing Tuwei Biotech Co.,Ltd.%College of Forestry and Life Sciences,Chongqing University of Arts and Sciences%Department of Plant Protection,Hebei North University
关键词：
soft rot pathogen;;housekeeping genes;;Dickeya;;MLSA;;minimal inhibit concentration
摘  要：
The pathogenicity of a pathogen isolated from the radish samples with the typical symptoms of soft rot in the Wuling Mountain in western Hunan was studied, and then the strain was identified by cultural and morphological characteristics, physicochemical tests, multilocus sequence analysis(MLSA) of 6 housekeeping genes(gapA, icdA, pgi, rpoS, mdh, proA). The results showed that the bacterial pathogen was Gram-negative, rod-shaped with strong pathogenicity of soft rot and infected various economic crops belonging to 13 genera in 8 families. The results showed that the strain shared a high similarity with Dickeya fangzhongdai. We determined the toxicity of 7 bactericides by the inhibition zone method. The results showed that agricultural streptomycin,SPP3 and SPP4 inhibited the growth of Secpp 1600 and there were significant differences among them in the inhibition rate. SPP4 showed the best direct antibacterial effect and the minimal inhibit concentration was 900 μg/mL.

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