中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 马铃薯Y病毒复制酶基因不同位置cDNA区段介导对PVY的不同抗性 植物保护学报 2010,37 (3) 227-233
Position: Home > Articles > Different virus-resistance to Potato virus Y infection mediated by different regions of NIb gene Journal of Plant Protection 2010,37 (3) 227-233

马铃薯Y病毒复制酶基因不同位置cDNA区段介导对PVY的不同抗性

作  者：
徐丽;宋云枝;王文俊;朱常香;温孚江
单  位：
山东农业大学;作物生物学国家重点实验室;山东农业大学植物保护学院;山东农业大学作物生物学国家重点实验室;山东农业大学生命科学学院
关键词：
马铃薯Y病毒;复制酶基因;RNA介导的病毒抗性
摘  要：
为探究靶序列位置对RNA介导的病毒抗性产生的影响,利用聚合酶链式反应(polymerase chain reaction,PCR)技术扩增马铃薯Y病毒(Potato virus Y,PVY)复制酶基因(nuclear inclusion b,NIb)不同位置的cDNA区段,反向插入双元载体pROKII中,构建了发夹RNA(hairpin RNA,hpR-NA)结构的植物表达载体。将构建的植物表达载体采用冻融法转入农杆菌LBA4404,叶盘法转化烟草NC89,获得转基因植株。攻毒试验表明:PVYNIb基因不同位置cDNA区段介导的对PVY的抗性存在显著差异;3′端1/2处和中间位置的序列可介导高水平的病毒抗性,抗性植株的比例在50%以上,而5′端、5′端1/2处和3′端的序列介导的抗性效率较低,抗性植株的比例仅为10%~30%。Northern杂交显示:抗病植株中RNA的积累量明显低于同类型的感病植株,抗性与RNA积累量呈负相关;抗病转基因植株中有siRNA存在,表明病毒抗性是由RNA介导的。
译  名：
Different virus-resistance to Potato virus Y infection mediated by different regions of NIb gene
作  者：
Xu Li1,2 Song Yunzhi2,3 Wang Wenjun3 Zhu Changxiang2,3 Wen Fujiang2,3(1.College of Plant Protection,Shandong Agricultural University,Tai'an 271018,Shandong Province,China;2.State Key Laboratory of Crop Biology,Shandong Agricultural University,Tai'an 271018,Shandong Province,China;3.College of Life and Sciences,Shandong Agricultural University,Tai'an 271018,Shandong Province,China)
关键词：
Potato virus Y;replicase gene;RNA-mediated virus resistance
摘  要：
In order to explore the impact of the location of target sequence on RNA-mediated virus resistance,different cDNA fragments of Potato virus Y(PVY) replicase gene(nuclear inclusion b,NIb) were amplified by polymerase chain reaction(PCR),and then inserted into plant binary vector pROKII.Hairpin RNAs(hpRNAs) expression vectors were constructed.The expression vectors were introduced into Agrobacterium tumefaciens strain LBA4404 by the frozen-throwing method,and eventually introduced into tobacco NC89 via leaf disk transformation.The results of virus challenge test showed that transgenic plants transformed sequences derived from different locations of NIb gene displayed different degrees of PVY resistance;the sequences derived from the 3′end 1/2 and the middle location mediated high levels of virus resistance,the proportion of resistant plants was above 50%;the sequences derived from the 5′end,5′end 1/2 and 3′end mediated low resistance efficiency,the proportion of resistant plants was only 10% to 30%.Northern blot showed that RNA accumulation in resistant plants was significantly lower than that in the susceptible plants,and there was a negative correlation between RNA accumulation and virus resistance,siRNAs were also detected in the resistant plants.These results indicated that the virus resistance was mediated by RNA.

相似文章

导出引用
计量
文章访问数: 20
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46