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当前位置: 首页 > 文章 > 芳香化酶抑制剂来曲唑对胡子鲇性腺分化及相关基因表达的影响 中国水产科学 2013,20 (5) 911-917
Position: Home > Articles > Effects of aromatase inhibitor letrozole on sex differentiation and related gene expression in Clarias fuscus Journal of Fishery Sciences of China 2013,20 (5) 911-917

芳香化酶抑制剂来曲唑对胡子鲇性腺分化及相关基因表达的影响

作  者：
李广丽;邓思平;孙晶;王文达;师尚丽;朱春华
单  位：
广东海洋大学
关键词：
胡子鲇;性分化;芳香化酶抑制剂;Cyp19a1b;Foxl2
摘  要：
采用组织学和荧光实时定量PCR方法,检测饲喂不同剂量芳香化酶抑制剂来曲唑(letrozole,50、100和200μg/g)对胡子鲇(Clarias fuscus)性腺组织学和性别比率的影响,以及200μg/g来曲唑对性分化前后脑型芳香化酶基因(Cyp19a1b)和翼状螺旋/叉头转录因子2(Foxl2)基因表达的影响,结果表明,50μg/g剂量的来曲唑对胡子鲇性腺分化无显著影响;但100μg/g和200μg/g剂量的来曲唑可促进胡子鲇精巢分化,抑制卵巢分化,卵巢腔最早出现时间和初级卵母细胞出现时间均分别推迟3 d和6 d,而初级精母细胞最早出现时间则分别提前2 d和5 d,且雄性率分别达65.8%和71.3%,显著高于50μg/L组和对照组(P<0.05)。胡子鲇性腺分化前(出膜后12 d),Cyp19a1b和Foxl2基因即开始表达,性腺分化前后Cyp19a1b相对表达量无显著差异(P>0.05),但Foxl2相对表达量则随性分化而逐渐降低,且来曲唑显著抑制性腺分化过程中Cyp19a1b和Foxl2的表达(P<0.05)。结果表明,100μg/g以上剂量的来曲唑可有效诱导胡子鲇分化为雄性;Cyp19a1b可能不直接参与胡子鲇性腺分化,但Foxl2直接参与此过程。
译  名：
Effects of aromatase inhibitor letrozole on sex differentiation and related gene expression in Clarias fuscus
作  者：
LI Guangli;DENG Siping;SUN Jing;WANG Wenda;SHI Shangli;ZHU Chunhua;Fisheries College, Guangdong Ocean University;Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Higher Education Institutes;
关键词：
Clarias fuscus;;sex differentiation;;aromatase inhibitor;;Foxl2;;Cyp19a1b
摘  要：
In this study, Clarias fuscus, a common freshwater fish in China, were selected to determine the effects of an aromatase inhibitor on sex differentiation and related gene expression. Two-day-old juvenile C. fuscus were fed different doses of the aromatase inhibitor, letrozole(50, 100, and 200 μg/g diets) for 30 days. The effects of letrozoleon sex ratio, gonad histology, and Foxl2 and Cyp19a1b expression were examined by morphological observation, histology, and real-time fluorescent quantitative PCR during the period of sex differentiation(12 30 d after hatching). The results showed that letrozole doses of 100 μg/g and 200 μg/g produced more males(65.9%and 71.3%, respectively) than did a dose of 50 μg/g(64.7%)(P<0.05). However, no significant difference in sex ratio was observed between the 50 μg/L 17α-MT treated group and the control group(51.1%). In addition, letrozole accelerated the occurrence of primary spermatocytes for 2 and 5 days, but deferred that of ovarian cavity and primary oocytes for 3 and 6 days, respectively. Both Cyp19a1b and Foxl2 were expressed prior to sex differentiation in C. fuscus. No difference was observed in the level of Cyp19a1b expression prior to and post sex differentiation(12 30 d after hatching). However, Foxl2 expression decreased with gonadal differentiation. In addition,letrozole at dose of 200 μg/g inhibited the expression of both Cyp19a1b and Foxl2(P<0.05). Our results suggest that letrozole doses above 100 μg/g could induce C. fuscus masculinization, and that Cyp19a1b is not directly involved in the process of gonadal differentiation but Foxl2 is.

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