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Position: Home > Articles > Bacterial Community Diversity of Moutai Distiller's Yeast Based on Metagenomics Guizhou Agricultural Sciences 2014 (11) 180-183+2

基于宏基因组学的茅台酒酒曲细菌的多样性分析

作  者:
唐婧;苏迪;徐小蓉;乙引
单  位:
贵州师范大学贵州省植物生理与发育重点实验室
关键词:
酒曲;宏基因组;细菌多样性;16SrDNA;茅台酒
摘  要:
为了解茅台酒酒曲微生物的菌群组成结构,为其制曲工艺的稳定及质量评估提供理论依据,利用宏基因组学方法分别提取2011—2013年的茅台酒酒曲微生物总基因组DNA,经PCR扩增16SrDNA V4区构建文库并测序,进行茅台酒酒曲细菌群落多样性的研究。结果表明:茅台酒酒曲微生物群落构成较稳定,主要分布于γ-变形菌纲(50%以上)和芽孢杆菌纲(30%以上),分属于魏斯氏菌属、片球菌属、明串球菌属、糖多孢菌属、欧文氏菌属、真丝菌属、短状杆菌属、鞘氨醇杆菌属、醋酸杆菌属、糖单孢菌属、盐单胞菌属和德库菌属;各年茅台酒酒曲细菌群落结构差异不大。
译  名:
Bacterial Community Diversity of Moutai Distiller's Yeast Based on Metagenomics
作  者:
TANG Jing;SU Di;XU Xiaorong;YI Yin;Guizhou Key Laboratory of Plant Physiology and Development,Guizhou Normal University;
关键词:
distiller's yeast;;bacterial diversity;;16SrDNA;;Moutai
摘  要:
Total DNA of microorganism in Moutai distiller's yeast with different years was respectively extracted by the metagenomics method and then its 16 SrDNA V4was amplified and sequenced to study bacterial community diversity of Moutai distiller's yeast for understanding composition and structure of bacterial community and provide the theoretical basis for stability and quality evaluation of distiller's yeast-making technology.The results showed that the microorganism community structure of Moutai distiller's yeast is relatively stable and the bacterial community mainly consists of Gammaproteobacteria(50%) and Bacilli(30%)including Weissella, Pedioccus, Leuconostoc,Saccharopolyspora,Erwinia, Planifilum,Brachybacterium sp.,Sphingobacterium, Acetobacter,Saccharomonospora,Halomonas and Desemzia.There is no difference in bacterial community structure among Moutai distiller's yeasts with different years.

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