中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 酸性蛋白酶制备花椒籽蛋白抗菌肽的研究 食品科学 2015,36 (13) 148-153
Position: Home > Articles > Preparation of Antibacterial Peptide Derived from Hydrolysis of Prickly Ash Seed Protein by Acid Protease FOOD SCIENCE 2015,36 (13) 148-153

酸性蛋白酶制备花椒籽蛋白抗菌肽的研究

作  者：
姜太玲;吴红洋;申光辉;董小华;张志清
单  位：
四川农业大学食品学院
关键词：
花椒籽蛋白;抗菌肽;酸性蛋白酶;抑菌率
摘  要：
利用酸性蛋白酶酶解花椒籽蛋白制备抗菌肽,以底物质量浓度、酶与底物比、p H值、酶解温度、酶解时间为影响因子,在单因素试验结果的基础上,应用Box-Behnken方法进行三因素三水平的试验设计,以对大肠杆菌的抑菌率为响应值,应用响应面法对花椒籽蛋白制备抗菌肽工艺进行优化。其最佳工艺条件为:底物质量浓度30 mg/m L、酶与底物比3.0%、酶解p H 4.0、酶解温度51.2℃、酶解时间4.7 h,此条件下酶解产生的抗菌肽复合物的水解度为9.05%,对大肠杆菌的抑菌率为56.98%。
译  名：
Preparation of Antibacterial Peptide Derived from Hydrolysis of Prickly Ash Seed Protein by Acid Protease
作  者：
JIANG Tailing;WU Hongyang;SHEN Guanghui;DONG Xiaohua;ZHANG Zhiqing;College of Food Science,Sichuan Agricultural University;
关键词：
prickly ash seed protein;;antibacterial peptide;;acid protease;;inhibitory rate
摘  要：
This work reports the preparation of antibacterial peptide by enzymatic hydrolysis of prickly ash seed protein with acid protease.Substrate concentration,enzyme/substrate ratio,p H,hydrolysis temperature and hydrolysis time were investigated by single factor method for their effect on the anti-E.coli activity of hydrolysates.Optimization of enzyme/substrate ratio,hydrolysis temperature and hydrolysis time based on anti-E.coli activity of hydrolysates was carried out using a Box-Behnken experimental design.The degree of hydrolysis of prickly ash seed protein was 9.05% and the inhibitory rate of the produced antibacterial peptide against E.coli was 56.98% when the hydrolysis experiment was carried out for 4.7 h at 51.2 ℃ and an initial p H of 4.0 with a substrate concentration of 30 mg/m L and an enzyme/substrate ratio of 3.0%.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46