作 者：
康元环;杨滨僮;孟庆峰;陈龙;单晓枫;王惠;钱爱东
单 位：
吉林农业大学动物科学技术学院;吉林出入境检验检疫局检验检疫技术中心
关键词：
不同宿主;维氏气单胞菌;OMPAⅡ基因;克隆
摘 要：
为探讨外膜蛋白AⅡ(OMPAⅡ)作为维氏气单胞菌共同保护性抗原的可能性,对不同宿主来源的维氏气单胞菌OMPAⅡ基因进行了克隆,获得了1 100bp左右的目的基因,并利用多种生物学软件对其序列进行了比较。结果表明,不同宿主来源的维氏气单胞菌OMPAⅡ基因序列的同源性在95.1%以上,编码氨基酸序列的同源性在97.3%以上,而且所有分析序列均具有明显的保守结构域,说明不同宿主来源的维氏气单胞菌OMPAⅡ具有很高的保守性;相对于ATCC35624和YP004393583.1来说,它们之间的差异主要表现在,CVCC3700、QXF、WL161、NJ34、NN725和BAF63175.1的OMPAⅡ基因缺失了3个碱基(CAA)。本研究结果为OMPAⅡ作为维氏气单胞菌基因工程亚单位疫苗候选成分提供了一定的理论依据。
译 名：
Cloning and comparison of OMPAⅡgenes of Aeromonas veronii from different hosts
作 者：
KANG Yuan-huan;YANG Bin-tong;MENG Qing-feng;CHEN Long;SHAN Xiao-feng;WANG Hui;QIAN Ai-dong;College of Animal Science and Technology,Jilin Agricultural University;Inspection and Quarantine Technology Center,Jilin Entry-Exit Inspection and Quarantine Bureau;
关键词：
different hosts;;Aeromonas veronii;;OMPAⅡgene;;cloning
摘 要：
To investigate the possibility of outer membrane protein AⅡ(OMPAⅡ)as a common protective antigen of Aeromonas veronii,A.veronii OMPAⅡgenes about 1 100bp from different hosts were obtained and the comparison and analysis were carried out between them by using several bio-software.The homology of these A.veronii OMPAⅡgenes were higher than 95.1% at the nucleotide level and higher than 97.3%at the amino acid level.And the same conserved domains were found,which showed that A. veronii OMPAⅡfrom different hosts had high-degree conservation.Difference between them was mainly manifested in that OMPAⅡgenes of CVCC3700,QXF,WL161,NJ34,NN725and BAF63175.1had the deletion of 3bases(CAA).This showed that OMPAⅡmight be considered as an important candidate for genetic engineering subunit vaccines against A.veronii.
