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当前位置: 首页 > 文章 > 多杀菌素发酵工艺的优化及其毒性初步研究 农药 2009,48 (10) 32-35
Position: Home > Articles > Optimization of Fermentation Process for Spinosad Production by Saccharopolyspora spinosa and Its Toxicity Agrochemicals 2009,48 (10) 32-35

多杀菌素发酵工艺的优化及其毒性初步研究

作  者：
朱明军;郑秋梅;胡飞;梁世中;杜顺堂
单  位：
华南理工大学;华粤行有限公司生物制约部
关键词：
多杀菌素;发酵工艺;毒性
摘  要：
对刺糖多孢菌S-6032发酵培养基及发酵工艺进行了优化,得到优化摇瓶发酵培养基为葡萄糖50g/L,麦芽糖10g/L,棉籽粉20g/L,硫酸铵1g/L,硫酸锌0.2g/L,玉米浆15g/L,牛肉膏2g/L,碳酸钙5g/L;确定摇瓶培养工艺为最佳接种量10%,最佳装液量为30mL/250mL摇瓶,最佳初始pH值为7.5。在优化培养条件下进行发酵培养,多杀菌素最终质量浓度达117.83mg/L,较优化前发酵培养条件下所得质量浓度(89.57mg/L)提高了31.6%。毒性实验表明发酵液对斜纹夜蛾幼虫毒杀效果显著,100mg/L多杀菌素的发酵液作用于斜纹夜蛾48h的致死率为90%。
译  名：
Optimization of Fermentation Process for Spinosad Production by Saccharopolyspora spinosa and Its Toxicity
作  者：
ZHU Ming-jun1a,ZHENG Qiu-mei1a,HU Fei1b,LIANG Shi-zhong1a,DU Shun-tang2(1.South China University of Technology a.School of Bioscience & Bioengineering,b.School of Light Industry & Food,Guangzhou 510640,China;2.Hua Yue Enterprise Holdings Ltd.,Guangzhou 511442,China)
关键词：
spinosad;fermentation process;toxicity test
摘  要：
Fermentation media and process for Saccharopolyspora spinosa S-6032 were optimized in the shake flask.The optimum media,which contained glucose 50 g/L,maltose 10 g/L,cottonseed powder 20 g/L,ammonium sulfate 1 g/L,zinc sulfate 0.2 g/L,corn steep liquor 15 g/L,beef extract 2 g/L and calcium carbonate 5 g/L,were obtained by orthogonal design experiment.And the optimum fermentation process,inoculum size of 10%,the liquid volume of 30 mL in the 250 mL shake flask and the initial pH of 7.5,were reached by single factor experiment.The ultimate production of 117.83 mg/L under the optimum conditions can be reached,which was increased by 31.6% compared to 89.57 mg/L on the original fermentation conditions.The toxicity test indicated that the broth with 100 mg/L spinosad had remarkable toxic effect on Prodenia liture(Fabricius).The lethal rate reached 90% after the pest was feeded with 100 mg/L spinosad for 48 h from the broth.

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