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当前位置: 首页 > 文章 > 电化学分析法测定植物油中的VE 食品科学 2008,29 (3) 369-373
Position: Home > Articles > Determination of Vitamin E in Vegetable Oils by Differential Pulse Voltammetry FOOD SCIENCE 2008,29 (3) 369-373

电化学分析法测定植物油中的VE

作  者：
李书国;陈辉;李雪梅;薛文通;张惠
单  位：
河北科技大学食品科学系;中国农业大学食品科学与营养工程学院
关键词：
VE;植物油分析;伏安技;电极
摘  要：
利用循环伏安技术研究了α-生育酚在玻碳电极上的伏安性质,其循环伏安图表明,在0.2～0.8V扫描电位范围内,在0.576V处出现一个良好的氧化峰,无还原峰出现,表明α-生育酚的电极反应是一个非可逆过程,是一个由扩散控制的电极反应过程。基于α-生育酚的电化学氧化机理,研究了α-生育酚的电化学分析法。利用微分脉冲伏安技术,研究确定了α-生育酚的最优分析条件:1,2二氯乙烷与乙醇(1:3)组成混合溶剂,支持电解质LiClO4的浓度为0.05mol/L,pH值为3.0,电位扫描速度为10mV/s,脉冲幅度为50mV;在5～350μmol/L范围内,α-生育酚的氧化峰电流与其浓度表现为线性关系,Ip=0.2872+0.01852×Cα-T,(相关系数r=0.9997),检测灵敏度为1.852×10-8AL/mol,最低检测为1.5μmol/L(S/N=3),每个样品分析所需时间80s。同时研究了植物油中常用合成抗氧化剂对α-生育酚的电化学法分析可能存在的影响与干扰,最后通过电化学分析法与HPLC法分析同一油样中VE的含量并进行对比,结果表明该法所测得的VE的含量与HPLC法测得的的α-、β-、γ-、δ-生育酚的总和一致,可用于植物油中VE的定量分析,无需任何油样预处理程序,操作简单,分析快捷、准确。
译  名：
Determination of Vitamin E in Vegetable Oils by Differential Pulse Voltammetry
作  者：
LI Shu-guo1,2,CHEN Hui2,LI Xue-mei2,XUE Wen-tong1,ZHANG Hui1 (1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2.Department of Food Science, Hebei University of Science and Technology, Shijiazhuang 050018, China)
关键词：
α-tocopherol;vegetable oil analysis;voltammetry;glass carbon electrode
摘  要：
The voltammetric behavior of α-tocopherol in the presence of vegetable oil has been invetigated at a glass carbon electrode in a 1,2 dichloroethane-ethanol medium with cyclic voltammetry. Cyclic voltammogram of α-tocopherol showed a well-defined oxidation peak at 0.576 V, no cathodic peak observed. The oxidation process of the α-tocopherol is a typical diffusion-controlled current process. The optimum analytical parameters were obtained: the mixture of 1,2-dichloroethane and ethanol (1:3) as solvent, 0.05 mol/L LiClO4 and pH 3.0, the instrumental parameters: 10 mV/s of scan rate and 50 mV of pulse height. Using differential pulse voltammetry, the peak currents were found to increase linearly with the α-tocopherol concentration over the range of 5.0 to 350μmol/L, with a sensitivity of 1.852×10-8 A L/mol and the limit of detection of 1.5μmol/L (S/N=3), the detection time being 80 s for each assay. The interference of other synthetic antioxidants such as TBHQ, BHA and BHT to the analysis of α-tocopherol was studied. The developed method is applied to the quantification of α-tocopherol in four vegetable oils, showing that the results are in agreement with the results by HPLC method. It could be used as a simple, rapid and practical method for the quantification of tocopherols in vegetable oils without any sample pretreatment.

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