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当前位置: 首页 > 文章 > 几种培养液对昆白系小鼠早期胚胎体外发育的影响 西北农业学报 2007,16 (3) 19-22+36
Position: Home > Articles > Effects of Several Media on the Early Embryonic Development of KM Strain Mouse in Vitro Acta Agriculturae Boreali-occidentalis Sinica 2007,16 (3) 19-22+36

几种培养液对昆白系小鼠早期胚胎体外发育的影响

作  者：
刘淑娟;华进联;葛秀国;严兴荣;胡勇策;窦忠英
单  位：
西北农林科技大学国家干细胞工程技术研究中心陕西分中心
关键词：
胚胎培养;细胞阻滞;KSOM;G1/G2
摘  要：
为了完善小鼠早期胚胎体外培养体系,探讨以KSOM为基础液添加新生牛血清(NBS)、胎牛血清(FBS)及BSA和G1/G2对小鼠早期胚胎体外发育的影响。结果表明:①添加10%NBS的KSOM组和G1/G2培养液组的囊胚率分别为89.52%和87.08%,二者差异不显著;②添加10%FBS组囊胚率为10.26%,与①中两组差异极显著;③添加0.1%BSA和0.4%BSA组囊胚率为0%,与其他三组差异极显著。添加新生牛血清的KSOM与胚胎培养液G1/G2均有效克服昆白小鼠2-细胞阻滞,并能得到较高的囊胚率。
译  名：
Effects of Several Media on the Early Embryonic Development of KM Strain Mouse in Vitro
作  者：
LIU Shu-juan,HUA Jin-lian,GE Xiu-guo,YAN Xing-rong,HU Yong-ce and DOU Zhong-ying (Northwest A﹠F University,Shaanxi Branch of National Stem Cells Engineering﹠Technology,Yangling Shaanxi 712100,China)
关键词：
Embryo culture;Cell block;KSOM;G1/G2
摘  要：
KSOM was used as a base medium.In order to improve the system for mouse embryo culture,the effects of adding newborn bovine serum(NBS),fetal bovine serum(FBS),bovine serum albumin(BSA) to KSOM respectively and G1/G2 medium on early mouse embryo development were studied.The result were as follows : ① The G1/G2 medium and KSOM containing 10% NBS could support mouse embryo development to blastocysts in 89.52% and 87.08% respectively.There were no differences between the two groups.② The blastocysts rate of KSOM with 10%FBS was 10.26%,significantly lower than group G1/G2 and KSOM containing 10%NBS.③ The rates of blastocysts of the two groups containing 0.4%BSA and 0.1%BSA were zero,significantly lower than other three groups.The KSOM medium with 10% NBS and medium G1/G2 were effective to overcome the 2-cell block,and the blastocysts rate of the two groups were high.

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