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Position: Home > Articles > Preliminary Studies on Early Genomic Changes of a Synthetic Allotetraploid in Cucumis Acta Horticulturae Sinica 2005,32 (6) 1105-1107

甜瓜属人工异源四倍体早期基因组变化的初步研究

作  者:
陈龙正;娄群峰;Joseph N.Wolukau;陈劲枫;耿红;罗向东
单  位:
南京农业大学园艺学院作物遗传与种质创新国家重点实验室
关键词:
甜瓜属;异源四倍体;AFLP分析;基因组序列变异
摘  要:
利用AFLP技术对甜瓜属人工异源四倍体(Cucum is×hytivusChen and K irkbride,2n=38)不同自交世代及二倍体亲本DNA进行分析,结果表明多倍体基因组在形成早期发生了广泛的变化,变化位点所占比例高达22.2%。基因组序列变化始于F1代,最高变化率出现在S2代。基因组变化主要表现为部分亲本片段丢失、新片段产生以及后代对亲本某些位点的跳跃式继承。还观察到部分双亲差异性条带呈现出偏父性遗传的特点。
译  名:
Preliminary Studies on Early Genomic Changes of a Synthetic Allotetraploid in Cucumis
作  者:
Chen Longzheng,Lou Qunfeng,Joseph N.Wolukau,Chen Jinfeng~*,Geng Hong,and Luo Xiangdong(College of Horticulture,Nanjing Agricultural University,National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing 210095,China)
关键词:
Cucumis;Allotetraploid;AFLP;Genomic change
摘  要:
AFLP analysis was carried out in amplification of the DNAs from different generations of the synthetic allotetraploid(Cucumis×hytivus,Chen and Kirkbride,2n=38),the interspecific F_1,and its diploid parents.The results indicated that extensive genomic changes occurred in the synthetic allotetraploid,and the variation rate was as high as 22.2%.We found the genomic changes started in the F_1 and the highest variation rate observed in S_2.Most genomic changes involved the loss of parental fragments,formation of novel fragments,with some fragments disappearing in one generation and reappearing in others.In addition,it was observed that some parent-specific bands were inherited preferentially from the male parent.

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