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当前位置: 首页 > 文章 > 毛地黄叶组织培养中光质对大分子物质含量及过氧化物酶同工酶酶谱的影响 上海交通大学学报(农业科学版) 1987 (4) 275-281
Position: Home > Articles > The Influence of Light Quality on Macromolecules Contents and Isozymes of peroxidase in Tissue Culture of Digitalis purpurea Journal of Shanghai Jiaotong University(Agricultural Science) 1987 (4) 275-281

毛地黄叶组织培养中光质对大分子物质含量及过氧化物酶同工酶酶谱的影响

作  者：
倪德祥;曹勇伟;张丕方;王凯基;潘重光
关键词：
光质;生物大分子;过氧化物酶同工酶;离体培养;毛地黄
摘  要：
光质不仅对毛地黄(Digitalis purpurea)叶愈伤组织的生长及器官发生有影响,同时也显著地影响了组织培养物中多糖类、蛋白质、DNA、叶绿素等生物大分子物质的含量。白光中蛋白质和叶绿素含量均较高,而蓝光、黑暗却抑制蛋白质的合成。生长值较高的绿光中含糖量较少,而生长值较低的黑暗中却有较多的糖。由此设想,光质对组织培养的影响与光合作用并无直接关系,光质可影响糖的转化过程,从而影响光形态建成。DNA 含量随光质的不同也有很大差异,并与生长值之间存在着显著的负相关。过氧化物酶活力测定表明,其比活力与形态发生有关,正处于器官发生高峰期的白光、黑暗、红光处理中,酶的比活力较高,而已有大量芽产生的黄、绿光处理组,其酶比活力较低。同工酶酶谱分析结果表明,黑暗中比有光条件下多一条 Px4谱带,各种光质既可影响到 Px1—3酶谱带的出现,也可影响各条带的宽窄和颜色深浅。这说明,光质对组织培养的影响,不但反映在基因的顺序表达水平上,而且也可作用在转录后的过程中。
译  名：
The Influence of Light Quality on Macromolecules Contents and Isozymes of peroxidase in Tissue Culture of Digitalis purpurea
作  者：
Ni Dexiang Cao Yongwei Zhang pifang Waljg Kaiji (Dept.of Biology,Fudan University) Pan Chongguang (Dept.of Horticulture,Shanghai Agricultural College)
关键词：
Light quality;;Macromolecules;;Peroxidase isoenzymes;;Cultured in vitro;;Digitalis purpurea
摘  要：
Light quality affected not only the callus growth and organogenesis but also the contents of carbohydrate,protein,DNA and chlorophyll.The highest protein and chlorophyll contents in white light were observed while blue light and dark inhibited protein synthesis.Tissue in green light was of a higher growth value but less carbohydrate content and it was just the opposite in dark.It is reasonable that the effects of light quality on plant tissue culture had no direct relationship with photosynthesis.The photomorphogenesis may be influenced by light quality through carbohydrate transformation.DNA contents also varied dramatically with light quality and showed a significant negative relationship with growth value. The specific activity of paroxidase was somehow related with the differentia- tion stage.Tissues of higher organogenesis activies in white,red light and dak were also of higher specific activities of the enzyme while the tissues in yellow and green light in which shoots had differentiated were of lower activities. The isozyme pattern of dank treatment contained an extra P_x4 band in comparing with light treatments.Light quality affected the strength of bands rather than the presence of bands P_x1 to3.This indicated that the effect of light quality on tissue culture occured not only in genes sequentis expression but also in pas- ttranscriptional process.

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