Characterization and Polymorphism of the 5′-flanking Region of Bovine Follicle-stimulating Hormone Receptor(FSHR) Gene

WEI Wu-chuan 1,XU Shang-zhong 2 (1.Academy of Animal and Veterinary Science,North-Western University of Agricultural and Forestry Science and Technology,Yangling,712100,China; 2.Institute of Animal Science,Chinese Academy of Agricultural Science,Beij

5′-flanking region of FSHR gene;Transcriptional promoter and regulating element;Polymorphism

931 bp,Including 134 bp of the first exon and 797 bp 5′-flanking region,of bovine FSHR gene was amplified by PCR using primers designed according to ovine FSHR gene sequence.The PCR products were ligated into PUC18 vector and sequenced.It was found that the genetic components of the bovine FSHR promoter differ from those of rat and human FSHR gene promoter.There are TATA-box and CAAT-box-like sequences in the promoter of bovine FSHR gene.Base-differences also exist at 5 loci of transcriptional regulating elements or characteristic sequences searched for,which may relate to the litter size differences of cattle and sheep.We also studied the polymorphism of the PCR products of 34 Chinese Simmental by PCR-RFLP.2 alleles and 3 genotype were verified by endonuclease Taq Ⅰ.Distributive differences of allele and genotype frequencies among herd-bull,twin-calved cow and random-sampling cattle indicates that these alleles are linked or correlated with the reproductive traits of cattle.