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广西部分地区猪细环病毒的分子流行病学调查

作  者:
洪绍锋;刘磊;刘欢;郭旋;黎木兰
单  位:
广西大学动物科学技术学院;广西大学动物科学技术学院广西丽原生物股份有限公司;广西大学动物科学技术学院广西兽医研究所广西农垦永新畜牧集团金光有限公司;广西大学;广西兽医研究所广西大学动物科学技术学院
关键词:
猪细环病毒;广西;分子流行病学;核苷酸同源性
摘  要:
为了解近年来才被发现的一种新型人畜共患DNA病毒——细环病毒在广西猪群中的流行情况,采用巢式PCR方法检测95份采自广西不同猪场、屠宰场的血清和组织样品(淋巴结、脾、肺),并挑选部分阳性样品进行基因克隆。结果显示,被检猪群中普遍存在细环病毒感染,TTSuV1和TTSuV2的阳性率分别为37.9%和20.0%,两者的混合感染率为11.6%。获得的9条TTSuV1和10条TTSuV2基因序列与NCBI上发布的参考序列同源性分别为81.8%~99.7%和79.2%~99.2%;遗传进化分析表明,广西猪群中流行的TTSuVs主要为TTSuV 1a、TTSuV 1b和TTSuV 2a。
译  名:
Molecular epidemiological investigation of Torque teno sus virus in part of Guangxi
作  者:
HONG Shao-feng1,LIU Lei1,LIU Huan1,GUO Xuan1,LI Mu-lan1,LU Bing-xia2, MO Yan-ning1,LONG Feng1,LIU De-qing3,HUANG Wei-jian1 (1.College of Animal Science and Technology,Guangxi University,Nanning 530005,China;2.Guangxi Veterinary Research Institute,Nanning 530001,China;3.Guangxi Reclamation Yongxin Animal Husbandry Group Jinguang Co.,Ltd.,Nanning 530042,China)
关键词:
Torque teno sus virus(TTSuV);Guangxi; molecular epidemiology;nucleotide homology
摘  要:
In order to know the prevalence and predominant genotype of Torque teno sus virus(TTSuV) found in recent years in Guangxi,95 blood and corresponding tissue samples(lymph node,spleen and lung) were collected from different pig farms in Guangxi and detected by a nested PCR method. Several positive samples were selected to clone and then sequenced. The results revealed that TTSuV infection was widespread in the detected pig population. The positive rates of TTSuV1 and TTSuV2 like viruses were 37.9% and 20.0% respectively,in which 11.6% were co-infected. The nucleotide sequences of the 9 TTSuV1 fragments and 10 TTSuV2 fragments were 81.8% to 99.7% and 79.2% to 99.2% identities,respectively when compared to those of the reference strains available in NCBI. Phylogenetic analysis showed the epidemic strains of TTSuVs in Guangxi were TTSuV 1a,TTSuV 1b and TTSuV 2a.

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