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当前位置: 首页 > 文章 > 离子液体-超声辅助萃取-高效液相色谱测定桑叶中黄酮 食品科学 2012,33 (24) 215-218
Position: Home > Articles > Ionic Liquid-based Ultrasonic-Assisted Extraction Coupled with HPLC for Sensitive Determination of Flavonoids in Mulberry Leaves FOOD SCIENCE 2012,33 (24) 215-218

离子液体-超声辅助萃取-高效液相色谱测定桑叶中黄酮

作  者：
陈君;周光明;杨远高;张丽贤
单  位：
西南大学化学化工学院发光与实时分析教育部重点实验室
关键词：
离子液体;超声辅助;高效液相色谱;黄酮;桑叶
摘  要：
目的:建立以离子液体1-丁基-3-甲基咪唑溴化盐([BMIM]Br)为萃取剂,结合超声辅助萃取,利用高效液相色谱法同时分离测定桑叶中的桑色素、槲皮素和山奈酚3种黄酮的方法。方法:采用Phenomenex C18色谱柱(250mm×4.6mm,5μm);流动相为甲醇-0.1%磷酸溶液,采用梯度洗脱;流速1.0mL/min;紫外检测波长365nm;柱温为34℃。结果:该方法对桑色素、槲皮素和山奈酚分别在0.0065～130、0.0064～128、0.0042～84μg/mL范围内线性关系良好,相关系数(r)均大于0.9998,检出限(RSN=3)依次为0.99、1.31、0.64ng/mL;样品回收率为90.00%～95.31%。结论:本法操作简单快速、定量准确、灵敏度高、成本低、且对环境友好,为桑叶中黄酮化合物的检测及分离提供了一个有效的科学方法。
译  名：
Ionic Liquid-based Ultrasonic-Assisted Extraction Coupled with HPLC for Sensitive Determination of Flavonoids in Mulberry Leaves
作  者：
CHEN Jun,ZHOU Guang-ming*,YANG Yuan-gao,ZHANG Li-xian(Key Laboratory on Luminescence and Real-Time Analysis,Ministry of Education,College of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China)
关键词：
ionic liquid;ultrasonic assistance;HPLC;flavonoids;mulberry leaves
摘  要：
An analytical method to determine flavonoids such as morin hydrate,quercetin and kaempferol in mulberry leaves was proposed using ultrasonic-assisted extraction coupled with HPLC.The extraction solvent used was 1-butyl-3methylimidazolium chloride([BMIM]Br).The chromatographic separation was performed on Phenomenex C18 column(250 mm × 4.6 mm,5 μm) using a mobile phase made up of methanol and 0.1% phosphoric acid at a flow rate of 1.0 mL/ min by gradient elution.The detection wavelength and column temperature were set as 365 nm and 34 ℃,respectively.This method showed good linearity in the range of 0.0065–130 μg/mL(r = 0.9999) for morin hydrate,of 0.0064–128 μg/mL(r = 0.9999) for quercetin,and of 0.0042–84 μg/mL for kaempferol.The limit of detection,LOD,(RSN = 3) of this method was 0.99,1.31 ng/mL and 0.64 ng/mL for morin hydrate,quercetin and kaempferol,respectively.Average recoveries ranging 90.00% to 95.31% were obtained for these three flavonoids(n = 3).This method was simple,fast,accurate,sensitive,cheap and environment-friendly and could provide a scientific way to determine flavonoids in mulberry leaves.

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