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当前位置: 首页 > 文章 > 日本血吸虫凋亡相关基因SjBAK的初步研究 中国动物传染病学报 2017 (3) 68-72
Position: Home > Articles > A PRELIMINARY STUDY ON APOPTOSIS ASSOCIATE GENES SJBAK OF SCHISTOSOMA JAPONICUM Chinese Journal of Animal Infectious Diseases 2017 (3) 68-72

日本血吸虫凋亡相关基因SjBAK的初步研究

作  者：
窦雪峰;王涛;吕超;韩倩;曹晓丹;沈元曦;张祖航;洪炀;傅志强;林矫矫
单  位：
上海师范大学生命与环境科学学院;中国农业科学院上海兽医研究所
关键词：
日本血吸虫;BAK;细胞凋亡
摘  要：
利用PCR技术扩增日本吸血虫凋亡相关基因BAK(Bcl-2 homologous antagonist/killer),并构建重组质粒。应用Realtime PCR技术分析在日本血吸虫非易感宿主大鼠和易感宿主小鼠来源、不同发育阶段虫体中BAK基因的表达状况。结果显示,克隆获得日本血吸虫(Schistosoma japonicum,Sj)BAK编码基因Sj BAK,其ORF含2142bp,编码713个氨基酸,理论分子量为79.3 k Da,理论等电点为4.9。结构域分析表明该基因编码蛋白具有至少3个BH结构域,属于Bcl家族成员。Real-time PCR结果显示该基因在大、小鼠来源4个不同发育阶段虫体均有表达,其中大鼠来源虫体Sj BAK的表达水平都高于小鼠来源虫体,在感染后32 d和42 d表达差异具有显著统计学意义(P<0.05)。由此可推测日本血吸虫凋亡相关基因Sj BAK在血吸虫生长发育中可能发挥重要的作用。
译  名：
A PRELIMINARY STUDY ON APOPTOSIS ASSOCIATE GENES SJBAK OF SCHISTOSOMA JAPONICUM
作  者：
DOU Xue-feng;WANG Tao;LV Chao;HAN Qian;CAO Xiao-dan;SHEN Yuan-xi;ZHANG Zu-hang;HONG Yang;FU Zhi-qiang;LIN Jiao-jiao;College of Life and Environmental Sciences, Shanghai Normal University;Shanghai Veterinary Research Institute,CAAS;
关键词：
Schistosoma japonicum;;BAK;;apoptosis
摘  要：
The objectives of the present study were to clone the apoptosis related gene of Schistosoma japonicum Sj BAK(Bcl-2 homologous antagonist/killer), analyze its expression in schistosome worms and possible role in the development of schistosome. The full-length sequence of Sj BAK was cloned in PCR and the recombinant plasmid containing Sj BAK was constructed. The expression of Sj BAK in S. japonicum at different development stages from non-susceptible host rats and susceptible host mice were analyzed using Real-time PCR. The ORF of the Sj BAK gene contained 2142 bp encoding 713 amino acids with the theoretical molecular weight of 79.3 k Da and theoretical isoelectric point at 4.9. Analysis also revealed that the molecule possessed at least three BH domains and belonged to the member of Bcl family. Real-time PCR analysis showed that the gene expressed in all worms at four development stages and the expression level in worms from rats was higher than those from mice. Significant difference in Sj BAK expression was observed in worms from two different hosts at 32 d and 42 d post-infection(P <0.05). In conclusion, the apoptosis-related gene Sj BAK of S japonicum may play an important role in the development of Schistosome.

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