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当前位置: 首页 > 文章 > 桑叶蛋白的提取及EDTA-2Na对其浸提过程的影响 中国农学通报 2014,30 (10) 57-61
Position: Home > Articles > Mulberry Leaf Protein Extraction and EDTA-2Na Affect Its Extraction Process Chinese Agricultural Science Bulletin 2014,30 (10) 57-61

桑叶蛋白的提取及EDTA-2Na对其浸提过程的影响

作  者：
杨田;张天宝;冀华;郑文雅;潘登奎
单  位：
山西农业大学文理学院
关键词：
桑叶;蛋白质;Na2HPO4-柠檬酸缓冲液;EDTA-2Na;蛋白酶抑制剂
摘  要：
为探索非变性原理提取桑叶蛋白的最佳条件,采用Na2HPO4-柠檬酸缓冲液提取桑叶蛋白,并进行蛋白质降解速度分析。通过4因素3水平正交试验对料液比、提取时间、提取温度、浸提液pH 4个因素进行优化,以叶蛋白得率、叶蛋白提取率为指标,得到提取桑叶蛋白的最佳工艺参数。结果表明,桑叶蛋白提取的优化工艺条件为:pH 7.6,料液比1:10,提取时间25 min,提取温度15℃。因新鲜叶片打浆时会破坏细胞壁,使细胞中的蛋白质在自溶酶的作用下发生分解,导致叶蛋白的提取率降低。为进一步提高桑叶蛋白的提取率,在最佳提取条件下,在打浆前向提取液中加入一定量的EDTA-2Na作为蛋白酶抑制剂,通过观察不同浓度EDTA-2Na作用下蛋白质的降解曲线,寻找EDTA-2Na的最适浓度,最终表明5 mmol/L的EDTA-2Na可最大限度降低浸提过程中蛋白质的降解。
译  名：
Mulberry Leaf Protein Extraction and EDTA-2Na Affect Its Extraction Process
作  者：
Yang Tian;Zhang Tianbao;Ji Hua;Zheng Wenya;Pan Dengkui;Collage of Arts and Science, Shanxi Agricultural University;
关键词：
mulberry leaf;;protein;;Na2HPO4-citric acid buffer;;EDTA-2Na;;protease inhibitor
摘  要：
In order to explore the optimum extracting conditions with non-denatured principle for leaf proteinfrom mulberry, use Na2HPO4-citric acid buffer to extract mulberry leaf protein and study the rate of proteindegradation. Through 4 factors and 3 levels orthogonal experiments, 4 factors including material-liquid ratio,extracting time, extracting temperature and pH value were optimized. Protein yield and protein extraction ratewere 2 indicators to find the most optimum processing parameters. The results showed that the most optimumconditions were as follows: pH 7.6, material-liquid ratio 1:10, extracting time 25 min, extracting temperature15℃. As soon as the author beated fresh leaves the cell walls must be destroyed, the protein in the cell mightdecompose by the action of the autolysis enzyme, which would lead to a lower leaf protein extraction rate.Under the optimum conditions the author added a certain amount of EDTA-2Na as a protease inhibitor beforebeating so as to enhance the rate of protein extraction. It was found this optimum concentration by observingthe protein degradation curve with different concentrations of EDTA- 2Na. Ultimate it showed that duringextraction, 5 mmol/L of EDTA-2Na could minimizes protein degradation.

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