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Position: Home > Articles > Bioinformatics Analysis and Function Prediction of a New Information-related Protein Gene BmP218 in Bombyx Mori Biotechnology Bulletin 2009,0 (1) 315-319

一种新的家蚕信息素相关蛋白基因BmP218的生物信息学分析及功能预测

作  者:
杨哲;高路;陈慧卿;陈克平
单  位:
江苏大学生命科学研究院
关键词:
家蚕;BmP281;幼虫;蜕皮激素;结合蛋白;NPV抗性
摘  要:
在进行抑制性减法杂交实验中,我们获得一条差异基因片段,EST拼接终获得了这一个654bp ORF阅读框, NCBI数据库检索未发现同源基因。但在家蚕数据库检索有同源基因存在为一种信息素相关的结合蛋白,PCR实验结果表明该基因确实在家蚕多种组织中存在转录,证明是一种新的基因,暂命名为BmP218。进一步生物信息学分析表明该定位在家蚕的第23号染色体上,有7个外显子。其同源物物有8个,虽然基因结构不同,但结构域非常相似。蛋白质分析中发现蛋白结构中包含有6个α螺旋,12个不规则卷曲,在氨基酸序列中存在有一段与昆虫细小病毒衣壳蛋白类似的区域(100-183)。
译  名:
Bioinformatics Analysis and Function Prediction of a New Information-related Protein Gene BmP218 in Bombyx Mori
作  者:
Yang Zhe Gao Lu Chen Huiqing Chen Keping (Institute of Life Sciences,JiangSu University,301 Xuefu Road,Zhenjiang 212013)
关键词:
Bombyx mori;;BmP218;;Larvae;;Ecdysone;;Binding protein;;NPV Resistance
摘  要:
After SSH experiment,we obtained a difference gene fragment,the EST splicing have obtained this 654bp ORF reading frame,and could not retrieval homologous gene in NCBI database.In Bombyx mori database searching,it's a kind of information element related binding protein contains 218 amino acids,PCR experimental result indicated that this gene expresses in many organizations of Bombyx mori,shows that it's a new gene in Bombyx mori,named BmP218.For further biological information analysis indicated that should locate in Bombyx mori 23rd chromosome,has 7 exons.There are about 8 homology proteins in Bombyx mori database,although the gene structure is different,but have a similar domain.Protein analysis shows that the protein structure contains 6α-helices,12 random coils,the amino acid sequences takes a similar region(100~183) to some insect parvovirus capsid protein.

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