当前位置: 首页 > 文章 > 利用病原真菌毒素离体筛选茄子抗黄萎病突变体的研究 华北农学报 2006,21 (1) 96-99
Position: Home > Articles > Research on in vitro Screening of Resistant Mutants of Eggplant to Verticillium daliae with Fungi Toxin Acta Agriculturae Boreali-Sinica 2006,21 (1) 96-99

利用病原真菌毒素离体筛选茄子抗黄萎病突变体的研究

作  者:
赵明敏;刘正坪;霍秀文
单  位:
北京农学院植科系;长江大学农学院;内蒙古农业大学农学院
关键词:
茄子黄萎病菌;毒素;离体筛选;抗病突变体
摘  要:
以茄子黄萎病菌毒素为选择剂,结合组织培养技术,离体筛选茄子抗黄萎病突变体,开拓一条体细胞抗病育种的新途径。结果表明,以茎段为外植体,在MS+IAA 1 mg/kg+NAA 1 mg/kg+KT 0.2 mg/kg培养基上愈伤组织诱导率最高,下胚轴次之,子叶最差;15%的毒素浓度(v/v)可以作为突变体的筛选压力;逐步提高毒素浓度能够在一定程度上提高愈伤组织对毒素的抗性。
译  名:
Research on in vitro Screening of Resistant Mutants of Eggplant to Verticillium daliae with Fungi Toxin
作  者:
ZHAO Ming-min~(1),LIU Zheng-ping~(2),HUO Xiu-wen~(3)(1.College of Agriculture,Yangtze University,Jingzhou 434025,China;2.Beijing Agricultural College,Beijing 102206,China;3.College of Agriculture,Inner Mongolia Agricultural University,Huhhot 010019,China)
关键词:
Verticillium daliae;Toxin;In vitro screening;Resistant mutant
摘  要:
In vitro screening of resistant mutants of eggplant to Verticillium daliae was studied with Verticillium daliae toxin and tissue culture technology.The induction rate in stem on medium of MS medium + IAA 1 mg/kg + NAA 1 mg/kg + KT 0.2 mg/kg was higher than that of in hypocotyl and cotyledons.The toxin concentration of 15% was considered as the selection pressure of resistant mutants of eggplant to Verticillium dahliae.The resistance of callus to Verticillium dahliae toxin could be improved by increasing the concentration gradually.

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