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当前位置: 首页 > 文章 > 浙江省绍兴市茄子黄萎病菌菌株致病型鉴定及其生物学特性研究 浙江农业学报 2019 (5) 784-789
Position: Home > Articles > 浙江省绍兴市茄子黄萎病菌菌株致病型鉴定及其生物学特性研究 Acta Agriculturae Zhejiangensis 2019 (5) 784-789

浙江省绍兴市茄子黄萎病菌菌株致病型鉴定及其生物学特性研究

作  者：
李戌清;张敬泽;张雅;吴根良
单  位：
杭州市农业科学研究院
关键词：
茄子黄萎病菌;致病型;特异性引物扩增;生物学特性
摘  要：
用大丽轮枝菌(Verticillium dahlida)落叶和非落叶致病型菌株特异性引物扩增,鉴定了浙江省绍兴市茄子黄萎病菌菌株的致病型,并研究了其生物学特性.特异性引物的聚合酶链式反应扩增结果表明,来自浙江省绍兴市的5个代表性分离菌株(VD-sy1、VD-sy2、VD-sy3、VD-sy4和VD-sy5)均属于落叶型菌株.生物学特性研究结果表明,菌株VD-sy1菌丝生长的最适条件为温度25℃,24 h黑暗,有较宽的酸碱度范围(pH 4.0~9.0);在以蔗糖为碳源和以酵母膏为氮源的培养基上菌丝生长较快,线性生长率分别为4.25和4.06 mm·d-1;在以半乳糖为碳源和以氯化铵为氮源的培养基上生长较慢,线性生长率分别为2.24和2.42 mm·d-1.
作  者：
LI Xuqing;ZHANG Jingze;ZHANG Ya;WU Genliang;Hangzhou Academy of Agricultural Sciences;College of Agriculture and Biotechnology, Zhejiang University;
单  位：
LI Xuqing%ZHANG Jingze%ZHANG Ya%WU Genliang%Hangzhou Academy of Agricultural Sciences%College of Agriculture and Biotechnology, Zhejiang University
关键词：
Verticillium dahliae;;pathotype;;specific primer amplification;;biological characteristics
摘  要：
Based on the method of polymerase chain reaction(PCR) amplification using the specific primers of defoliation and nondefoliation pathotypes of Verticillium dahliae, the pathotype of the isolates causing the verticillium wilt disease of eggplant in Shaoxing City of Zhejiang Province were identified. And the biological characteristics of V. dahliae were also studied. Results of PCR amplification showed that the isolates(VD-sy1, VD-sy2, VD-sy3, VD-sy4 and VD-sy5) obtained all belonged to the defoliating pathotype. The results of biological characteristics showed that VD-sy1 was able to grow at pH between 4.0 to 9.0, and 25 ℃ under 24 h dark condition was beneficial to colony growth; sucrose and yeast extract were the best carbon and nitrogen source for mycelia with linear growth rates of 4.25 and 4.06 mm·d~(-1), respectively, while galactose and ammonium chloride showed the least liner growth rates of 2.24 and 2.42 mm·d~(-1), respectively.

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