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减蛋综合征病毒(EDSV)的分离与鉴定

作  者:
王雪敏
单  位:
邯郸农业高等专科学校动科系
关键词:
减蛋综合征病毒;分离;鉴定
摘  要:
从河北邯郸某鸡场190d未经EDS疫苗免疫的、突然产蛋下降并出现大量软壳蛋、无壳蛋的鸡群中分离到一株病毒,经理化特性鉴定、单抗Dot-ELISA检测、DNA探针斑点杂交,确定分离到的病毒为EDSV。经限制性核酸内切酶酶切分析,表明分离株与EDSV标准株AV127属于同一个基因型。
译  名:
Isolation and Identification of Egg Drop Syndrome Virus(EDSV)
作  者:
Wang Xuemin(Department of Animal Science and Technology of Handan Agricultural College,Yongnian Hebei057150)
关键词:
egg drop syndrome virus;isolation;identification
摘  要:
One virus strain was isolated from190-day-old poultry.The infected chickens showed egg drop with soft-shelled and shellless eggs.By identifying physicochemical properties,using McAb Dot-ELISA and DNA probe Dot-biot,the strain was identified as an Egg drop syndrome virus(EDSV).DNAs of the isolate were analysed by using different restriction endonucleases.The result showed that the isolate could belong to the same genotype as the standard strain AV127.

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