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当前位置: 首页 > 文章 > 山豆根组培苗的质量分析 北方园艺 2012 (13) 185-187
Position: Home > Articles > Analysis on the Ouality of Tissue-cultured Plantlets of Sophora tonkinensis Gapnep. Northern Horticulture 2012 (13) 185-187

山豆根组培苗的质量分析

作  者：
韦坤华;李林轩;王美英;张占江;缪剑华
单  位：
广西药用植物园;广西药用植物资源保护与遗传改良重点实验室
关键词：
山豆根;苦参碱;氧化苦参碱;组培苗
摘  要：
以山豆根组培生根苗为试材,采用TLC法鉴别其是否存在苦参碱和氧化苦参碱,并使用HPLC法测定其组培苗移栽前后苦参碱和氧化苦参碱的含量。结果表明:山豆根组培生根苗含有苦参碱和氧化苦参碱,且与氧化苦参碱和苦参碱对照品色谱相同的位置上,显示相同的橙红色斑点;HPLC定量分析结果表明,山豆根组培苗移栽田间4个月含有的苦参碱和氧化苦参碱均低于田间生长6个月的山豆根实生苗。
译  名：
Analysis on the Ouality of Tissue-cultured Plantlets of Sophora tonkinensis Gapnep.
作  者：
WEI Kun-hua,LI Lin-xuan,WANG Mei-ying,ZHANG Zhan-jiang,MIAO Jian-hua(Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement,Guangxi Botanical Garden of Medicinal Plants,Nanning,Guangxi 530023)
关键词：
Sophora tonkinensis Gapnep.;matrine;oxymatrine;tissue-cultured plantlet
摘  要：
With Sophora tonkinensis Gapnep tissue culture seedling as material,the presence of matrine and oxymatrine in the tissue-cultured plantlets of Sophora tonkinensis Gapnep.were identified by TLC,and also the matrine and oxymatrine contents of tissue-cultured plantlets before and after transplanted were determinated by HPLC.The results showed that there were reddish orange spots at the corresponding place of matrine and oxymatrine.The analysis of HPLC showed that both of the tissue-cultured plantlets before transplanted and after transplanted in the field for four monthes possessed matrine and oxymatrine,but lower than the seedlings from seeds planted in the field for half year.This study demonstrates that the tissue-cultured plantlets of S.tonkinensis Gapnep.contain matrine and oxymatrine,and it will provide a good support for follow-up research.

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