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当前位置: 首页 > 文章 > AT毒素胁迫的再生烟草及后代对赤星病的抗性 植物保护学报 1994,21 (4) 321-326
Position: Home > Articles > RESISTANCE TO BROWN SPOT IN TOBACCO REGENERANTS PRODUCED BY SOMACLONE STRESS WITH AT-TOXIN Journal of Plant Protection 1994,21 (4) 321-326

AT毒素胁迫的再生烟草及后代对赤星病的抗性

作  者：
董汉松;卢培秀;刘爱新;孙衍坤
单  位：
山东农业大学植物保护系
关键词：
烟草赤星病;诱导抗性;AT毒素;再生植株;NC89—TT
摘  要：
从4个烤烟品种幼苗心叶叶碟产生的愈伤组织(CA)在含AT毒素的培养基上经两轮筛选,获得的抗毒素CA用于产生了抗病的一次再生植株。用来自抗病性提高50％以上的一次再生株的二代CA产生了抗病的二次再生植株,品种NC89的二次再生抗病植株被称为NC89—TT。获得种子后,NC89—TT二代苗显示出抗毒素损伤、抗病菌侵入、抗病斑扩展的能力,与NC89比,毒素引起的根和叶部病变至少晚8小时、轻64％,病菌孢子侵染率低60％以上,病斑面积减小92％。
译  名：
RESISTANCE TO BROWN SPOT IN TOBACCO REGENERANTS PRODUCED BY SOMACLONE STRESS WITH AT-TOXIN
作  者：
Dong Hansong Lu Peixiu Liu Aixin Sun Yankun (Department of Plant Protection, Shandong Agricultural University, Tai' an)
关键词：
tobacco brown spot induced resistance AT-toxin . regen- erated plants somaclone NC89-TT
摘  要：
For improving tobacco resistance to brown spot through somaclonal induction under the pressure of AT-toxin of Alternaria alternata, three cultivars of flue-cured tobacco which are excellent in quality properties but susceptible to the disease in China, were selected as target materials. A native cv, ZY86 ,which is undesirable in quality but more resistant to the disease was used as reference material. The first clones of regenerants were obtained from the toxin-resistant calluses resulted from 2 selection circulations of AT-toxin stress culture. The second clones of calluses from regenerated plants of first clones showed resistance to AT-toxin at rates of 19% -80% and 14% -69%, respectively. When the toxin contents in media were 60% and 75%. The second clones of regenerated plants occurred at a 100% resistant rate in cv NC89. The best second clone of regenerated plants of NC89 was named as NC89-TT. Seedling from NC89-TT progeny gave 64% and 92% reduction of damage by AT-toxin and infection by spores of the pathogen.

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