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当前位置: 首页 > 文章 > 高效液相色谱荧光检测法测定虾中的4-己基间苯二酚 食品科学 2009,30 (14) 238-239
Position: Home > Articles > Determination of 4-Hexylresorcinol in Shrimp by HPLC with Fluorescence Detection FOOD SCIENCE 2009,30 (14) 238-239

高效液相色谱荧光检测法测定虾中的4-己基间苯二酚

作  者：
段小娟;蔡发;牟志春;王曼霞
单  位：
山东出入境检验检疫局检验检疫技术中心
关键词：
4-己基间苯二酚;虾;高效液相色谱
摘  要：
目的:建立快速检测虾中4-己基间苯二酚(4-HR)的高效液相色谱法。方法:用乙腈提取虾中的4-HR后,以反相C18柱为分离柱,以甲醇-水溶液为流动相洗脱,采用高效液相色谱-荧光检测器,于激发波长280nm,发射波长320nm定量检测。结果:4-HR在10min内出峰,线性范围为0.02～50.0mg/L(r=0.99996),检出限为0.1mg/kg,回收率为97.6%～99.9%,相对标准偏差(RSD)为2.1%～4.6%。结论:本法准确、可靠、简便、检出限低,适合分析大批量样品。
译  名：
Determination of 4-Hexylresorcinol in Shrimp by HPLC with Fluorescence Detection
作  者：
DUAN Xiao-juan,CAI Fa,MOU Zhi-chun,WANG Man-xia (Technology Center of Inspection and Quarantine, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China)
关键词：
4-hexylresorcinol;shrimp;high performance liquid chromatography
摘  要：
A rapid HPLC method was developed to determine 4-hexylresorcinol (4-HR) in shrimp meat. The sample was extracted with acetonitrile, filtrated through the 0.45μm membrane and then injected into the HPLC column. The analysis was performed on a reversed-phase C18 column using a mobile phase consisting of methanol and water (70:30, V/V) with fluorimetric detection (excitation: 280 nm, and emission: 320 nm). The analyte could be separated within 10 min by the procedure. The linear range was 0.02-50.0 mg/L (r = 0.99996) and the detection limit was 0.1 mg/kg. The average recovery ranged from 97.6% to 99.9% (n = 7), with a relative standard derivation from 2.1% to 4.6%. The method has the advantages of simplicity, repeatability and low detection limit, and can be used for analysis of shrimp samples in large quantity.

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