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当前位置: 首页 > 文章 > 两种特异性氰戊菊酯人工抗原的合成与鉴定 食品科学 2011,32 (7) 178-182
Position: Home > Articles > Synthesis and Identification of Two Types of Specific Artificial Antigen for Fenvalerate FOOD SCIENCE 2011,32 (7) 178-182

两种特异性氰戊菊酯人工抗原的合成与鉴定

作  者：
熊振宇;杨艳燕;李培武;张奇;张文;丁小霞
单  位：
湖北大学生命科学学院;中国农业科学院油料作物研究所
关键词：
氰戊菊酯;半抗原;人工抗原;合成
摘  要：
选择化学结构具有典型研究意义的氰戊菊酯(fenvalerate,Fen)为研究对象,利用有机化学合成法在氰戊菊酯分子的一端分别引入两种不同连接长度的连接臂。通过核磁共振(1H-NMR)、液相色谱-质谱联用仪(LC-MS-MS)鉴定,成功合成两种半抗原Fen1和Fen2,并与牛血清蛋白(bovine serum albumin,BSA)偶联合成了两种氰戊菊酯免疫特异性人工抗原Fen1-BSA、Fen2-BSA,经紫外分光光度仪扫描,人工抗原的紫外图谱相对于半抗原和载体蛋白有明显差异,测得偶联比分别约为9:1和10:1,经SDS-PAGE电泳检测表明偶联成功。
译  名：
Synthesis and Identification of Two Types of Specific Artificial Antigen for Fenvalerate
作  者：
XIONG Zhen-yu1,YANG Yan-yan1,LI Pei-wu2,3,4,ZHANG Qi2,3,4,ZHANG Wen2,3,4,DING Xiao-xia2,3,4(1.Faculty of Life Sciences,Hubei University,Wuhan 430062,China;2.Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China;3.Key Laboratory of Oil Crops Biology,Ministry of Agriculture,Wuhan 430062,China;4.Quality Inspection and Test Center for Oilseeds and Products,Ministry of Agriculture,Wuhan 430062,China)
关键词：
fenvalerate;hapten;artificial antigen;synthesis
摘  要：
Fenvalerate,with a typical chemical structure,was selected as the research object in this study.Two types of haptens were synthesized through separately introducing two space arms with different connecting lengths to one end of fenvalerate by organic synthesis.1H-NMR and LC-MS-MS identifications demonstrated the successful synthesis of haptens,which were designated as Fen 1 and Fen 2.Fen 1 and Fen 2 were separately coupled to bovine serum albumin(BSA),and two kinds of specific immunogens were obtained and named as Fen 1-BSA and Fen 2-BSA.UV-visible spectroscopic analysis indicated that the two artificial immunogens differed significantly from their corresponding heptens and the protein carrier.The coupling ratios between Fen 1 or Fen 2 and BSA were around 9:1 and 10:1,respectively.The results of SDS-PAGE showed that both haptens were conjugated to BSA successfully.

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