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当前位置: 首页 > 文章 > 花生油和玉米油中多组分真菌毒素高效液相色谱-串联质谱检测方法的建立 食品科学 2017,38 (10) 297-304
Position: Home > Articles > Development of High Performance Liquid Chromatography-Tandem Mass Spectrometry for Detection of Multi-Mycotoxins in Peanut Oil and Corn Oil FOOD SCIENCE 2017,38 (10) 297-304

花生油和玉米油中多组分真菌毒素高效液相色谱-串联质谱检测方法的建立

作  者：
刘丹;韩小敏;李凤琴;王畅;罗晓林;卢大新
单  位：
中山大学公共卫生学院;国家食品安全风险评估中心;北京农学院食品科学与工程学院
关键词：
真菌毒素;花生油;玉米油;高效液相色谱-串联质谱
摘  要：
建立花生油和玉米油中黄曲霉毒素、玉米赤霉烯酮、单端孢霉烯族化合物等13种真菌毒素的高效液相色谱-串联质谱检测方法。样品经乙腈-水提取、Multi Sep 226多功能净化柱净化后,分别用电喷雾正离子模式和负离子模式检测。经优化,正离子模式流动相为含2 mmol/L甲酸铵的0.1%甲酸-甲醇溶液,负离子模式流动相为0.1%氨水-乙腈溶液。该方法对花生油和玉米油中13种真菌毒素的检出限为0.05~3.00μg/kg,定量限为0.10~10.00μg/kg,平均加标回收率为66.6%~114.9%。所建方法操作简单、灵敏度高、重复性好,可用于花生油和玉米油中多组分真菌毒素的协同测定。
译  名：
Development of High Performance Liquid Chromatography-Tandem Mass Spectrometry for Detection of Multi-Mycotoxins in Peanut Oil and Corn Oil
作  者：
LIU Dan;HAN Xiaomin;LI Fengqin;WANG Chang;LUO Xiaolin;LU Daxin;College of Food Science and Engineering,Beijing University of Agriculture;Beijing Key Laboratory of Agricultural Product Detection and Control for Spoilage Organisms and Pesticides;Key Laboratory of Food Safety Risk Assessment,Ministry of Health,China National Centre for Food Safety Risk Assessment;School of Public Health,Sun Yat-Sen University;
关键词：
mycotoxin;;peanut oil;;corn oil;;high performance liquid chromatography-tandem mass spectrometry
摘  要：
A high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) method was developed for the simultaneous detection of 13 mycotoxins including aflatoxins, zearalenone, and trichothecenes in peanut and corn oils. Samples were extracted with acetonitrile-water, followed by clean-up with a Multi Sep 226 multifunctional column. The positive electrospray ionization mode(ESI+) and negative electrospray ionization mode(ESI-) were respectively employed to acquire signals. The mobile phase for ESI+ was composed of 0.1% formic acid containing 2 mmol/L ammonium formate(solvent A) and methanol(solvent B), while that for ESI-consisted of 0.1% ammonia water(solvent A) and acetonitrile(solvent B). The limits of detections(LODs) for 13 mycotoxins in peanut and corn oils ranged from 0.05 to 3.00 μg/kg and the limits of quantification(LOQ) ranged from 0.10 to 10.00 μg/kg. The mean recoveries ranged from 66.6% to 114.9%. The method developed in the present study had the advantages of simple operation, high sensitivity and good reproducibility, and so it could be used for the detection of multi-mycotoxins in peanut and corn oils.

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