中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 砀山酥梨~(60)Coγ射线辐射诱变效应及其RAPD标记检测 安徽农业大学学报 2010,37 (3) 172-177
Position: Home > Articles > Effect of ~(60)Co γ-ray radiation on mutation of Chinese pear cultivar Dangshansuli and its detection by RAPD marker Journal of Anhui Agricultural University 2010,37 (3) 172-177

砀山酥梨~(60)Coγ射线辐射诱变效应及其RAPD标记检测

作  者：
王妍炜;叶振风;衡伟;朱立武
单  位：
安徽农业大学果树学重点实验室
关键词：
梨;60Coγ射线;萌芽率;新梢生长量;RAPD标记
摘  要：
为探索辐射对砀山酥梨新梢生长和诱变效应的影响,探讨分子标记辅助辐射诱变育种新技术,利用砀山酥梨休眠枝条进行60Coγ射线辐照处理,枝条高接后分别对萌芽率、新梢生长量进行统计。提取不同处理叶片基因组DNA,利用RAPD分子标记分析处理剂量对基因组DNA变异的影响。结果表明,30 Gy、40 Gy和50 Gy处理的萌芽率分别为0.725 7、0.542 7和0.194 0,新梢生长量分别为48.21、35.47和25.67 cm。从42个随机引物中筛选出10条适宜引物对材料变异进行分析,发现随辐射剂量的增加,萌芽率、新梢生长量随之降低,分子检测变异程度随之增大。
译  名：
Effect of ~(60)Co γ-ray radiation on mutation of Chinese pear cultivar Dangshansuli and its detection by RAPD marker
作  者：
WANG Yan-wei,YE Zhen-feng,HENG Wei,ZHU Li-wu(Key Laboratory of Pomology,Anhui Agricultural University,Hefei 230036)
关键词：
pear;60Co γ-ray;bud survival rate;length of shoot growth;RAPD marker
摘  要：
To study the effect of irradiation on the shoot growth and mutation of pear and discuss the technology of the molecular marker-assisted mutagenic breeding,the dormant branch of Chinese pear cultivar Dangshansuli was treated with 60Co γ-ray irradiation and than grafted.The survival rate of treated bud and the length of shoot growth were investigated.The relationship between different treatment doses and the mutagenic rates detected in level of genomic DNA,which were extracted from the leaves by CTAB method,was also analyzed by RAPD marker.The results showed that the survival rates of bud treated with the dose of 30 Gy,40 Gy and 50 Gy were 0.7257,0.5427,0.1940,of which the length of shoot growth was 48.21,35.47 and 25.67 cm,respectively.Ten suitable random primers were screened out from 42 ones.Therefore,it was proposed that the survival rate of bud and the length of shoot growth were decreased as the irradiation dose increased.The degree of variation was increased as the irradiation dose increased.

相似文章

导出引用
计量
文章访问数: 15
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46