单 位：
西北农林科技大学葡萄酒学院;徐州工程学院江苏省食品资源开发与质量安全重点建设实验室
摘 要：
通过超滤与大孔树脂吸附分离黑豆蛋白酶解产物,获得降胆固醇活性较强的黑豆肽组分,采用凝胶过滤色谱(GFC)、反相高效液相色谱(RP-HPLC)与排阻色谱(SEC)对黑豆肽粗品进行纯化,得到高纯度的降胆固醇肽,经Edman降解、N端测序对降胆固醇黑豆肽一级结构进行分析鉴定,其氨基酸组成序列为Ala-Phe-Pro-Lys-Asp,相对分子质量为576,肽链中具有特定的疏水区域,且Pro位于N端之外的重要位点。
译 名：
Isolation and Identification of Hypocholesterolemic Peptide Derived from Black Soybean Protein
作 者：
LIU En-qi;LI Hua;WU Yong-hua;GAO Zhao-jian;ZHANG Jian-ping;College of Enology,Northwest A&F University;Jiangsu Key Laboratory of Food Resource Development and Quality Safety,Xuzhou Institute of Technology;
关键词：
black soybean peptides;;hypocholesterolemic activity;;isolation;;structural analysis
摘 要：
Black soybean peptides(BSPs) with strong hypocholesterolemic activity were purified from black soybean protein hydrolysates by using ultrafiltration(UF) and macroporous adsorption resin(MAR), and further purified using Sephadex G-25 column, reversed phase high-performance liquid chromatography(RP-HPLC) and size exclusion chromatography(SEC). The amino acid sequence of the peptide with the strongest hypocholesterolemic activity was confirmed as Ala-Phe-Pro-Lys-Asp by automated Edman degradation in a protein sequencer, and its relative molecular mass was determined to be 576 D. In addition, a specific hydrophobic domain in the sequence was observed, and Pro was located in an important region except the N-terminus.
