单 位：
西北农林科技大学葡萄酒学院;徐州工程学院;山西农业大学食品科学与工程学院
关键词：
黑豆肽;抗氧化;小鼠;缓解体力疲劳;氨基酸组成
摘 要：
通过超滤与大孔吸附树脂分离黑豆蛋白酶解产物,获得体外抗氧化活性较强的黑豆肽,测定其相对分子质量分布和氨基酸组成;将小鼠随机分为空白对照组、谷胱甘肽(GSH)阳性对照组和黑豆肽低、中、高剂量组5组,进行缓解体力疲劳动物实验.结果表明:相对分子质量<3000、以体积分数75%乙醇洗脱的黑豆肽具有相对最强的DPPH自由基清除率;黑豆肽中、高剂量组小鼠负重游泳时间、血乳酸和肝糖原含量与空白组差异极显著(P<0.01),但达到缓解体力疲劳同等效果的灌胃剂量约是GSH的5~10倍;抗氧化黑豆肽的相对分子质量约在450~920之间,具有Phe、Val、Pro、Met疏水性氨基酸和Thr、Cys、His等含量较高的氨基酸组成特点,可以延缓由自由基引发的疲劳.
单 位：
College of Enology,Northwest A&F University,Yangling 712100,China%Jiangsu Key Laboratory of Food Resource Development and Quality Safety,Xuzhou Institute of Technology,Xuzhou 221008,China%College of Food Science and Engineering,Shanxi Agricultural University,Jinzhong 030801,China
关键词：
black soybean peptides%antioxidant capacity%mice%physical fatigu-relieving function%amino acid composition
摘 要：
Black soybean peptides(BSPs) with potent antioxidant activity,purified with ultrafiltration and macroporous adsorption resin(MAR) from black soybean protein hydrolysates,were analyzed for relative molecular mass and amino acid composition.A total of 125 mice were randomly divided into control group which were given normal saline,positive group which were given reduced glutathione,and low,medium and high dose groups which were given BSPs for 30 days,then the weight loaded-swimming time and blood lactic acid(BLA),serum urea nitrogen(SUN) and hepatic glycogen(HG) contents were observed for evaluating the fatigue-relieving function of BSPs.The results showed that the peptide fraction with relative molecular mass < 3000 eluted by 75% ethanol displayed the highest DPPH radical-scavenging activity.Compared with the control group,the middle and high dose groups showed significantly(P < 0.01) prolonged loaded-swimming time,lowered BLA,and improved HG storage status.This indicates that oral administration of BSPs can relieve physical fatigue in mice.The effect of the low dose group(250 mg/(kg d)) on BLA and HG and the effect of the middle dose group(500 mg/(kg d)) on loaded-swimming time were similar to that of the positive group(50 mg/(kg d)).BSPs with relative molecular mass between 450 and 920 were obtained from further separation by Sephadex G-25 gel chromatography,which possessed high contents of Phe,Val,Pro,Met,Thr,Cys and His,displaye better antioxidant activity,and also could relieve physical fatigue triggered by free radicals.
