中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > A new gain-of-function OsGS2/GRF4 allele generated by CRISPR/Cas9 genome editing increases rice grain size and yield 作物学报(英文版) 2022,10 (4)
Position: Home > Articles > A new gain-of-function OsGS2/GRF4 allele generated by CRISPR/Cas9 genome editing increases rice grain size and yield The Crop Journal 2022,10 (4)

A new gain-of-function OsGS2/GRF4 allele generated by CRISPR/Cas9 genome editing increases rice grain size and yield

作  者：
Wenshu Wang;Weipeng Wang;Yanlin Pan;Chao Tan;Hongjing Li;Chen Ya;Xingdan Liu;Jing Wei;Nian Xu;Yu Zhu Han;Huiying Gu;Rongjian Ye;Qi Ding;Castello M
单  位：
State Key Laboratory of Crop Breeding Technology Innovation and Integration, China National Seed Group Co., Ltd., Wuhan 430000, Hubei, Chin;Life Science and Technology Center, China National Seed Group Co., Ltd., Wuhan 430000, Hubei, China; State Key Laboratory of Crop Breeding Technology Innovation and Integration, China National Seed Group Co., Ltd., Wuhan 430000, Hubei, China
关键词：
Genome editing;GS2/GRF4;Grain size;Yield;Ric
摘  要：
Grain size is one of the most important factors affecting rice grain quality and yield, and attracts great attention from molecular biologists and breeders. In this study, we engineered a CRISPR/Cas9 system targeting the miR396 recognition site of the rice GS2 gene, which encodes growth-regulating factor 4 (OsGRF4) and regulates multiple agronomic traits including grain size, grain quality, nitrogen use efficiency, abiotic stress response, and seed shattering. In contrast to most previous genome editing efforts in which indel mutations were chosen to obtain null mutants, a mutant named GS2(E) carrying an in-frame 6-bp deletion and 1-bp substitution within the miR396-targeted sequence was identified. GS2(E) plants showed increased expression of GS2 in consistent with impaired repression by miR396. As expected, the gain-of-function GS2(E) mutant exhibited multiple beneficial traits including increased grain size and yield and bigger grain length/width ratio. Thousand grain weight and grain yield per plant of GS2(E) plants were increased by 23.5% and 10.4%, respectively. These improved traits were passed to hybrids in a semi-dominant way, suggesting that the new GS2(E) allele has great potential in rice improvement. Taken together, we report new GS2 germplasm and describe a novel gene-editing strategy that can be widely employed to improve grain size and yield in rice. This trait-improvement strategy could be applied to other genes containing miRNA target sites, in particular the conserved miR396-GRF/GIF module that governs plant growth, development and environmental response. (C) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

相似文章

PDF下载 PDF预览
导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46