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当前位置: 首页 > 文章 > Exploiting viral vectors to deliver genome editing reagents in plants 生物技术通报(英文版) 2024,5 (2)
Position: Home > Articles > Exploiting viral vectors to deliver genome editing reagents in plants aBIOTECH 2024,5 (2)

Exploiting viral vectors to deliver genome editing reagents in plants

作  者：
Yilin Shen;Ye Tao;Zihan Li;Torotwa Herman Kimutai;Hao Song;Oliver Xiaoou Dong;Jianmin Wa
关键词：
CRISPR/Cas;Genome editing;Plant genome engineering;Virus-based deliver
摘  要：
Genome editing holds great promise for the molecular breeding of plants, yet its application is hindered by the shortage of simple and effective means of delivering genome editing reagents into plants. Conventional plant transformation-based methods for delivery of genome editing reagents into plants often involve prolonged tissue culture, a labor-intensive and technically challenging process for many elite crop cultivars. In this review, we describe various virus-based methods that have been employed to deliver genome editing reagents, including components of the CRISPR/Cas machinery and donor DNA for precision editing in plants. We update the progress in these methods with recent successful examples of genome editing achieved through virus-based delivery in different plant species, highlight the advantages and limitations of these delivery approaches, and discuss the remaining challenges.

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