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当前位置: 首页 > 文章 > 5株氟罗沙星单克隆抗体杂交瘤细胞株的品质评价 中国农学通报 2009,25 (24) 96-101
Position: Home > Articles > Quality Evaluation on the Monoclonal Antibodies of Against Fleroxacin of Five Hybridoma Cells Chinese Agricultural Science Bulletin 2009,25 (24) 96-101

5株氟罗沙星单克隆抗体杂交瘤细胞株的品质评价

作  者：
谭成成;刘艳霞;李晓艳;万忠海
单  位：
解放军军事医学科学院军事兽医研究所;金宇保灵生物药品有限公司;吉林大学畜牧兽医学院
关键词：
氟罗沙星;单克隆抗体;品质评价
摘  要：
通过对5株杂交瘤细胞株分泌稳定性、抗体亚型、亲和力、特异性等方面的测定,表明其染色体众数为87～90条,具有众数的细胞所占比例为81%～84%,单抗亚型均为IgG1型,腹水单抗的亲和常数为108～109M-1,氟罗沙星单抗与诺氟沙星、环丙沙星、恩诺沙星、链霉素、庆大霉素、卡那霉素的交叉反应试验表明,所制备的抗氟罗沙星单抗对氟喹诺酮类药物有较高的交叉反应率,与链霉素、庆大霉素、卡那霉素无交叉反应。
译  名：
Quality Evaluation on the Monoclonal Antibodies of Against Fleroxacin of Five Hybridoma Cells
作  者：
Tan Chengcheng1, Liu Yanxia2, Li Xiaoyan3, Wan Zhonghai3(1Pasturage and Theriatrics School, Jilin University, Changchun 130062; 2Ginyu Baoling Biological Drug Limited Company,Hohhot 010030; 3Institute of Military Veterinary Academy of Military Medical Science, PLA, Changchun 130062)
关键词：
fleroxacin, McAb, quality evaluation
摘  要：
The quality of the prepared anti-Fleroxacin monoclonal antibodies were evaluated by the stability of secretion, antibody-subtype, affinity and specificity experiments' results. It showed that the five strains of hybridoma cells contained modal number of chromatin for 87-90 which occupied for 81%-84%, subtypes of monoclonal antibodies were all IgG1 types, affinity constant of ascites monoclonal antibodys was 108-109M-1.The cross-reaction experiments of Fleroxacin monoclonal antibody were carried out with Norfloxacin, Ciprofloxacin, Enrofloxacin, Streptomycin, Gentamicin, Kanamycin. From the experiments we know that the prepared anti-fleroxacin monoclonal antibodies had a very high reaction rate with fluoroquinolones, but had no reaction with streptomycin, gentamicin, kanamycin.

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