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当前位置: 首页 > 文章 > 巴马小型猪主要固有免疫分子的克隆及序列分析 黑龙江畜牧兽医 2014 (21) 13-16+21
Position: Home > Articles > Cloning and sequence analysis of the main innate immune molecules in Bama miniature pig Heilongjiang Animal Science and Veterinary Medicine 2014 (21) 13-16+21

巴马小型猪主要固有免疫分子的克隆及序列分析

作  者：
胡晓亮;姜骞;郭东春;刘家森;仇铮;林欢;刘培欣;田进;李志杰;曲娟娟;曲连东
单  位：
中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室;东北农业大学资源与环境学院;东北农业大学动物医学学院
关键词：
巴马小型猪;固有免疫;克隆;序列分析
摘  要：
为了开展巴马猪的固有免疫相关研究,试验采用巴马小型猪的外周血分离淋巴细胞,提取总RNA,利用特异性引物进行RT-PCR,将克隆片段与pc DNA3.1载体相连接,转化到大肠杆菌DH5α中,筛选阳性克隆并进行测序,并将测序结果提交NCBI,比较克隆序列与已知序列的同源性。结果表明:克隆的巴马猪TLR7、TLR8、TLR9、VISA、STING和IRF3序列长度分别为3 075,3 087,3 097,1 575,1 260,1 137 bp,测序结果与预期相符,各序列与猪的同源性较高。
译  名：
Cloning and sequence analysis of the main innate immune molecules in Bama miniature pig
作  者：
HU Xiao-liang;JIANG Qian;GUO Dong-chun;LIU Jia-sen;QIU Zheng;LIN Huan;LIU Pei-xin;TIAN Jin;LI Zhi-jie;QU Juan-juan;QU Lian-dong;National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences;College of Resources and Environment,Northeast Agricultural University;College of Veterinary Medicine,Northeast Agricultural University;
关键词：
Bama miniature pig;;innate immune;;cloning;;sequence analysis
摘  要：
To carry out the related research of innate immunity in Bama miniature pig,the lymphocytes were separated from the peripheral blood of Bama miniature pig,and then were used to extract the total RNA. The specific primers were used for RT-PCR,and the cloned fragments were connected with pc DNA3. 1 vector,and then were transformed into E. coli DH5α. The positive clones were screened,and then the sequencing results were submitted to the NCBI,for comparing the homology between the cloned sequences and the known sequences. The results showed that the sequence lengths of the TLR 7,TLR 8,TLR 9,VISA,STING and IRF3 genes from Bama miniature pig were 3075 bp,3087 bp,3097 bp,1575 bp,1260 bp and 1137 bp,respectively. The sequencing results were in line with the expected lengths of target genes.Through sequence analysis,it was found that the sequence homology of these molecules had high homology with that of pigs.

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