作 者：
汪宏才;商雨;郭云清;曾哲;张蓉蓉;温国元;罗青平
关键词：
水禽细小病毒;新型鹅细小病毒;分离鉴定;选择压力;基因重组
摘 要：
通过对临床水禽细小病毒进行PCR检测、基因组测序和进化分析,确定分离毒株的基因型.进一步通过Paml、RDP软件对分离株的基因组进行选择压力和基因重组分析,确定分离株的演化趋势.结果表明,分离的3株水禽细小病毒均属于新型鹅细小病毒,分别命名为YiCH株、AnQ株和XiY株,基因组长度分别为5 056、5 056、5 068 bp.3个分离株与新型鹅细小病毒M15株亲源关系最近,其中XiY株两端ITR区域191~196位、4 878~4 883位均有6个碱基插入.对分离株进行选择压力分析,分离株VP蛋白检测出3个正选择位点,分别为116Q、261A、485S.重组分析显示,分离株YiCH株和AnQ株都存在重组现象,以XiY株为主要亲本株、GPV 06-0329株为次要亲本株重组而成.
译 名：
Selection pressure and gene recombination analysis of waterfowl parvovirus
关键词：
waterfowl parvovirus%novel goose parvovirus%isolation and identification%select pressure%gene recombination
摘 要：
By conducting PCR detection,genome sequencing,and evolutionary analysis on clinical waterfowl parvovirus,the geno-type of the isolated strain was determined.Further selection pressure and gene recombination analysis were conducted on the genome of the isolated strains using Paml and RDP software to determine the evolutionary trend of the isolated strains.The results showed that the three strains of waterfowl parvovirus isolated belonged to the novel goose parvovirus,named YiCH strain,AnQ strain,and XiY strain,with genome lengths of 5 056,5 056,and 5 068 bp,respectively.The three isolates had the closest phylogenetic relationship with the novel goose parvovirus M15 strain,with 6 base insertions at positions 191~196 and 4 878~4 883 in the ITR region at both ends of the XiY strain.Selection pressure analysis was conducted on the isolated strains,and three positive selection sites were detected for the VP protein of the isolated strains,namely 116Q,261A,and 485S.Recombination analysis showed that both the YiCH and AnQ strains exhibited recombination phenomenon,with XiY strain as the main parent strain and GPV 06-0329 strain as the secondary par-ent strain.
