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Position: Home > Articles > Isolation and Assaying of Polysaccharide in Zizyphus jujuba Mill cv.lingwuchangzao Northern Horticulture 2011 (14) 43-45

灵武长枣多糖含量测定的研究

作  者:
杨军;章英才;苏伟东;杨淑娟
单  位:
宁夏大学生命科学学院;宁夏红枣工程技术研究中心
关键词:
灵武长枣;多糖;苯酚-硫酸比色法;含量测定
摘  要:
灵武长枣样品经脱脂,脱小分子糖后,用蒸馏水沸水浴浸提、醇沉、洗涤得灵武长枣多糖,含量测定采用苯酚-硫酸比色法,用精制多糖测得其多糖对葡萄糖的换算因子。建立了一种简便、准确测定灵武长枣多糖含量的方法。结果表明:该方法测定简便可行,重现性较好,平均回收率为97.11%,所测全红灵武长枣样品中多糖含量为13.30%。
译  名:
Isolation and Assaying of Polysaccharide in Zizyphus jujuba Mill cv.lingwuchangzao
作  者:
YANG Jun1,SU Wei-dong2,YANG Shu-juan1,ZHANG Ying-cai1(1.College of Life Science,Ningxia University,Yinchuan,Ningxia 750021;2.Ningxia Jujube Engineering Technology Research Center,Yinchuan,Ningxia 750021)
关键词:
Zizyphus jujuba Mill cv.lingwuchangzao;polysaccharide;phenol-sulfuric acid colorimetry;determination
摘  要:
With ungrease treatment and oligo-saccharide,and extract by boiling water-bath,ethanol precipitation,deprivate protein,and determinated its content by phenol-sulfuric acid method.This experiment established a simple,accurate determination method of polysaccharide form Zizyphus jujuba Mill cv.lingwuchangzao.The results showed that it was a simple,feasible method to determinate polysaccharide content,the recovery was 97.11%,the polysaccharide in Zizyphus jujuba Mill cv.lingwuchangzao was 13.30%.

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