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Position: Home > Articles > Embryogenic Callus Induction and Plant Regeneration of Zoysia japonica Acta Horticulturae Sinica 2003,30 (3) 355-357+380

结缕草愈伤组织诱导及植株再生

作  者:
李瑞芬;张敬原;赵茂林
单  位:
北京市农林科学院北京农业生物技术研究中心
关键词:
结缕草;胚性愈伤;组织培养;植株再生
摘  要:
以日本结缕草种子为外植体 ,2份材料在附加 2 4 D 2 0mg/L水平的MS培养基上愈伤组织诱导率较高 ;在含不同水平 2 4 D的培养基中添加 6 BA对非胚性愈伤组织转变成胚性愈伤组织起重要作用 ,其中 2 4 D 2 0mg/L配合 6 BA 0 1mg/L的MS培养基诱导出的胚性愈伤组织比例较高 ;胚性愈伤组织在 2 4 D为 0 1mg/L的分化培养基上分化率和生根率最高 ,为 46 8%~ 48 1%。
译  名:
Embryogenic Callus Induction and Plant Regeneration of Zoysia japonica
作  者:
Li Ruifen,Zhang Jingyuan,and Zhao Maolin (Beijing Academy of Agricultural and Forestry Sciences,Beijing Agro-biotechnology Research Center,Beijing 100089,China)
关键词:
Zoysia japonica Steud.;Embyrogenic callus;Tissue culture;Plant regeneration
摘  要:
Mature caryopses of two materials of Zoysia japonica Stend.collected in different habitats were used as initial explants for callus induction.MS containing 2.0 mg/L 24-D induced calli at the relatively higher percentage of 31.2%-42.1%.Inclusion of 6-BA in callus induction medium with various levels of 24-D made an important role for non-embryogenic calli changing into embryogenic calli.The embryogenic calli of higher percentage were induced by a combination of 2.0 mg/L 24-D and 0.1 mg/L 6-BA.The rates of shoot regeneration and rooting were different in different regeneration medium.The rates of shoot regeneration and rooting were 46.8%-48.1% at the level of 0.1 mg/L 24-D.

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