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当前位置: 首页 > 文章 > 狗牙根内生固氮菌对结缕草的促生效应 北方园艺 2017 (13) 113-118
Position: Home > Articles > Promoting Effect of Endophytic Diazotrophs From Bermudagrass on Zoysia japonica Steud Northern Horticulture 2017 (13) 113-118

狗牙根内生固氮菌对结缕草的促生效应

作  者：
武鑫;李凤娇;刘天增;张巨明
单  位：
华南农业大学草业工程技术研究中心;华南农业大学林学与风景园林学院
关键词：
内生固氮菌;接种;结缕草;促生作用
摘  要：
以结缕草为试材,将分离自野生狗牙根的内生固氮菌DQ-3活化测定活性后制成2种浓度菌液(1×10~3、1×10~6 cfu·mL~(-1)),再将长势相同的结缕草茎段于菌液中浸泡4、16h后进行盆栽试验,采用测定成坪后植株的颜色、分蘖数、地上地下生物量及植株全氮量的方法,研究内生固氮菌对结缕草的促生效果,以探索不同草种间内生固氮菌的通用性。结果表明:菌液浓度为1×10~6 cfu·mL~(-1),浸泡4h对结缕草分蘖数促进最显著;菌液浓度为1×10~6 cfu·mL~(-1),浸泡16h对颜色的影响最大;菌液浓度为1×10~6 cfu·mL~(-1),浸泡16h对地上生物量影响最大;菌液浓度为1×10~3 cfu·mL~(-1),浸泡4h对地下生物量影响最大;菌液浓度为1×10~6 cfu·mL~(-1),浸泡16h对结缕草植株增加全氮量效果最好。综合看来,菌液浓度为1×10~6 cfu·mL~(-1),浸泡16h的组合对结缕草促生效果最好。
译  名：
Promoting Effect of Endophytic Diazotrophs From Bermudagrass on Zoysia japonica Steud
作  者：
WU Xin;LI Fengjiao;LIU Tianzeng;ZHANG Juming;College of Forestry and Landscape Architecture,South China Agricultural University;Engineering Research Center of Grassland Science,South China Agricultural University;
关键词：
endophyte diazotroph;;inoculation;;Zoysia japonica Steud;;promoting effect
摘  要：
Taking Zoysia japonica Steud as test materials,the endophyte diazotroph QD-3was isolated from bermudagrass and made into two different concentration bacteria liquid(1×10~3 cfu·mL~(-1) and 1×10~6 cfu·mL~(-1)).After soaking Zoysia japonica Steud stems with similar growth for 4hours and 16 hours respectively,the potting test began.When turf established,the turf color,tillers number,aboveground biomass,underground biomass and total nitrogen were evaluated to study the promoting effect of endophytic diazotrophs on zoysia grass.In the meanwhile,the versatility between different grass species could also be explored.The results showed that soaking under the concentration of 1×10~6 cfu·mL~(-1) for 4hours had the most promoted tillers number;soaking under the concentration of 1×10~6 cfu·mL~(-1) for 16 hours had well-influenced turf color;soaking under the concentration of 1×10~6 cfu·mL~(-1) for 16 hours showed the most improved aboveground biomass while soaking under the concentration of 1×10~3 cfu·mL~(-1) for 4hours had greater underground biomass.Soaking under the concentration of 1×10~6 cfu·mL~(-1) for 16 hours had the best promotion in total nitrogen of Zoysia japonica Steud.In conclusion,soaking under the concentration of 1×10~6 cfu·mL~(-1) for 16 hours had the best promoting effect on Zoysia japonica Steud.

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