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SiO_2球腔微电极阵列过氧化氢传感器制备及应用

作  者:
李建鑫;尹凡;吴向阳;吴静萍;徐玉兰
单  位:
苏州大学药学院;江苏大学化学与化工学院;江苏省新型功能材料重点建设实验室
关键词:
过氧化氢;溶胶-凝胶法;二氧化硅球腔;微电极阵列;微过氧化物酶-11;生物传感器
摘  要:
将微过氧化物酶-11(MP-11)直接固定于二氧化硅球腔微电极阵列,制备一种新型电化学过氧化氢(H2O2)生物传感器。采用Langmuir-Blodgett技术在氧化铟锡(ITO)电极表面制备聚苯乙烯(PS)微球阵列,并以此阵列为模板采用溶胶-凝胶法在ITO电极上制备二氧化硅(SiO2)球腔阵列,最后将微过氧化物酶-11作为氧化还原模型蛋白直接吸附于球腔内,制得MP-11/SiO2球腔阵列/ITO电极。该电极对H2O2响应快速灵敏,可作为电流型H2O2电化学生物传感器,其线性范围为7.06×10-6~4.02×10-2mol/L,检出限为3.0×10-7mol/L,米氏常数为0.916mmol/L。将该法用于食品样品中的H2O2的检测,回收率在94%~97%之间,效果良好,可为食品中残存的H2O2检测提供一种方法。
译  名:
Preparation and Application of Hydrogen Dioxide Biosensor Based on Microelectrode Array of SiO_2 Cavity
作  者:
LI Jian-xin1,YIN Fan2,*,WU Xiang-yang1,WU Jing-ping2,3,XU Yu-lan2(1.College of Chemistry and Chemical Engineering,Jiangsu University,Zhenjiang 212013,China;2.Jiangsu Laboratory of Advanced Functional Materials,Changshu 215500,China;3.College of Pharmaceutical Sciences,Soochow University,Suzhou 215123,China)
关键词:
hydrogen peroxide;sol-gel technique;silicon dioxide cavity;microelectrode array;microperoxidase-11;biosensor
摘  要:
A new biosensor for the detection of H2O2was prepared by immobilizing microperoxidase-11(MP-11) onto silicon dioxide(SiO2) cavity array directly.Polystyrene(PS) particle array was constructed on indium-tin oxide(ITO) electrode surface by Langmuir-Blodgett technique.The silicon dioxide cavity array was prepared by Langmuir-Blodgett technique using PS particle array as the template.Using MP-11 as a model protein,MP-11/SiO2 cavity array-ITO electrode was prepared by adsorbing MP-11 into SiO2cavity.The fast response of the biosensor against H2O2was observed.A linear relationship between current response and H2O2 concentration ranging from 7.06 × 10-6 to 4.02 × 10-2 mol/L was achieved with a detection limit of 3.0 × 10-7 mol/L.The apparent Michaelis-Menten constant was 0.916 mmol/L.This established method is satisfactory for the determination of hydrogen peroxide in food samples with a recovery rate varying from 94% to 97%.

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