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Position: Home > Articles > Regeneration Plants of Cotyledon and Hypocotyl of Xinjiang Brassica rapa L. Northern Horticulture 2012 (24) 132-135

新疆恰玛古子叶及下胚轴植株再生体系的建立

作  者:
地丽热巴·地里夏提;木合热皮亚·艾尔肯;张富春;张霞
单  位:
新疆大学生命科学与技术学院;新疆生物资源基因工程重点实验室
关键词:
恰玛古;子叶;下胚轴;愈伤组织;再生植株
摘  要:
以新疆恰玛古种子萌发5d无菌苗的子叶及下胚轴为外植体,研究了各种不同激素配比条件下新疆恰玛古的再生体系。结果表明:培养基MS+2,4-D 2.0mg/L+KT 0.1mg/L对愈伤组织诱导效果较好,子叶、下胚轴的愈伤诱导率分别为65.4%、95.5%;再生芽的诱导培养基为6-BA 3.0mg/L+NAA 0.5mg/L+AgNO35.0mg/L,子叶、下胚轴的芽分化率分别为20.8%、65.0%。将芽转入生根培养基MS+IBA 0.5mg/L中诱导生根。20d后,生根的小植株练苗移入花土中,成活率达95%。
译  名:
Regeneration Plants of Cotyledon and Hypocotyl of Xinjiang Brassica rapa L.
作  者:
Dilraba·DILXAT,Muhrapiya·ARKIN,ZHANG Fu-chun,ZHANG Xia(Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi,Xinjiang 830046)
关键词:
Brassica rapa L.;cotyledon;hypocotyl;callus;regeneration plants
摘  要:
The regeneration system of tissue culture of Xinjiang Brassica rapa L.was discussed by using cotyledon and hypocotyl of 5 day-old seedling of Xinjiang Brassica rapa.L as the explant.The results showed that MS+2,4-D 2.0 mg/L+KT 0.1 mg/L medium was effective for callus induced,the averaging callus frequences were 65.4%,95.5% for cotyledon,hypocotyl;shoots were induced with 6-BA 3.0 mg/L+NAA 0.5 mg/L+AgNO3 5.0 mg/L,the averaging regeneration shoots frequence was 20.8%,65.0% for cotyledon,hypocotyl;shoots was transferred into medium of MS+IBA 0.5 mg/L to root;after 20 days,regeneration plants were transferred into flower-soil and its survivable rate was more than 95%.

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