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当前位置: 首页 > 文章 > 太平洋鳕仔鱼期消化酶活性的变化 广东海洋大学学报 2013,33 (1) 33-37
Position: Home > Articles > Development of Digestive Enzymes in Gadus macrocephalus Larvae Journal of Guangdong Ocean University 2013,33 (1) 33-37

太平洋鳕仔鱼期消化酶活性的变化

作  者：
宫雪;姜志强;吴洪;范瑞;李艳秋
单  位：
大连海洋大学农业部北方海水增养殖重点实验室
关键词：
太平洋鳕;仔鱼;酸性蛋白酶;胰蛋白酶;淀粉酶;脂肪酶
摘  要：
用酶学分析法研究太平洋鳕仔鱼发育过程中胰蛋白酶、酸性蛋白酶、淀粉酶和脂肪酶活性的变化。结果表明:4种酶的比活力均在初孵时即可检出,但比活力变化趋势不同;胰蛋白酶比活力在仔鱼孵化初期较高,随后显著降低;酸性蛋白酶比活力在早期仔鱼阶段比较低,且较为稳定,从14日龄开始显著升高;淀粉酶比活力在3日龄即达到最大值,之后显著降低,并一直保持较低的比活力;脂肪酶的比活力一直较低,在5日龄达到最大值,之后比活力大幅度下降。仔鱼发育过程中,各种酶活性变化显著(p<0.05),太平洋鳕在仔鱼期已经可以消化蛋白质、脂肪和碳水化合物。
译  名：
Development of Digestive Enzymes in Gadus macrocephalus Larvae
作  者：
GONG Xue,JIANG Zhi-qiang,WU Hong,FAN Rui,LI Yan-qiu(Key Laboratory of Mariculture& Stock Enhancement in North China’s Sea,Ministry of Agriculture,Dalian Ocean University,Dalian,116023,China)
关键词：
Gadus macrocephalus;larva;acid protease;trypsin;amylase;lipase
摘  要：
The development of digestive enzymes was examined in laboratory-reared larvae of Gadus macrocephalus from 0 to 30 days after hatch(DAH).The trypsin,acid protease,amylase and lipase activities of the larvae were investigated.The total activities of these digestive enzymes were detected,and showed a gradual increase from 0 to 30 DAH,but the activities of these enzymes present different trends.The specific activities of trypsin showed the maximum on 4 DAH followed by a quick decline and then stayed at low level from 5 to 30 DAH.The enzyme activities of acid protease stayed at low and stable level from 0 to 12 DAH and began to significantly increase from 14 DAH.The specific activities of amylase showed the maximum on 3 DAH,and followed by a quick decline and stayed at low level.The specific activities of lipase showed the similar trend as amylase.The maximum of lipase appeared on 5 DAH.Significant variations(p < 0.05) in specific activities of all the enzymes were found.The composition of digestive enzymes indicates that the larvae of Gadus macrocephalus are able to digest protein,lipid and carbohydrates at an early stage.

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