关键词：
氧化魔芋葡甘露聚糖;齐口裂腹鱼;品质;过氧化物酶体增殖物激活受体-α;脂肪酸结合蛋白
摘 要：
目的:研究日粮氧化魔芋葡甘露聚糖(oxidized konjac glucomannan,OKGM)对齐口裂腹鱼的品质和脂肪代谢相关基因表达的影响。方法:将初始体质量为80 g的齐口裂腹鱼随机分成5组,分别饲喂质量分数为0(对照组)、0.4%、0.8%、1.6%、3.2%OKGM,养殖60 d后,考察OKGM对齐口裂腹鱼的品质和脂肪代谢相关基因表达的影响。结果:与对照组相比,日粮OKGM添加量为1.6%和3.2%能显著增加齐口裂腹鱼腹肌中蛋白质含量(P<0.05),添加量为0.8%时,会显著降低腹肌粗脂肪含量(P<0.05),OKGM添加量对齐口裂腹鱼肌肉中水分和粗灰分无显著影响(P>0.05);添加量对齐口裂腹鱼肌肉的pH值、失水率、羟脯氨酸含量、胶原蛋白含量的影响均无显著性差异(P>0.05);添加量为1.6%时能显著升高肌肉中过氧化物酶体增殖物激活受体-α(peroxisome proliferator activated receptor-α,PPAR-α)mRNA表达水平(P<0.05),添加量为0.8%能显著升高肝胰脏中PPAR-αmRNA表达水平(P<0.05);添加量为0.8%能极显著降低齐口裂腹鱼肌肉中脂肪酸结合蛋白(fatty acid binding protein,FABP)mRNA表达水平(P<0.01),添加量对肝胰脏中FABP mRNA表达水平的影响差异均不显著(P>0.05)。结论:日粮中添加适量OKGM能改善齐口裂腹鱼的品质,调节脂肪代谢相关基因表达。
译 名：
Effect of Dietary Oxidized Konjac Glucomannan on Quality and Lipid Metabolism-Related Gene Expression of Schizothorax prenanti Tchang
作 者：
ZHOU Cheng;WU Ying-long;XIANG Shi-qiong;XIA Xiao-jie;College of Food Science, Sichuan Agricultural University;
关键词：
oxidized konjac glucomannan;;Schizothorax prenanti Tchang;;quality;;peroxisome proliferator activated receptor-α;;fatty acid-binding protein
摘 要：
Objective: To study the effect of dietary oxidized konjac glucomannan(OKGM) on the quality and lipid metabolismrelated gene expression of Schizothorax prenanti Tchang(SPT). Methods: The SPT with body weight of approximately 80 g were randomly divided into five groups which were fed 0(control group), 0.4%, 0.8%, 1.6% and 3.2%(experimental groups) OKGM for 60 days, respectively. The results showed that compared with the control group, the protein content in abdominal muscle of SPT was significantly increased by adding 1.6% and 3.2% OKGM in the SPT diet(P < 0.05), the fat content in abdominal muscle was significantly reduced by adding 0.8% OKGM in the diet(P < 0.05); however, the amount of OKGM had no significant impact on muscle moisture or crude ash in SPT(P > 0.05). In addition, no significant difference in pH, dehydration rate, hydroxyproline content or collagen content in abdominal muscle of SPT was observed in response to varying amounts of OKGM(P > 0.05). Meanwhile, PPAR-α mRNA expression level in muscle of SPT was significantly increased by dietary addition of 1.6% OKGM(P < 0.05), and a significant increase in this parameter was also observed for liver of SPT administered with 0.8% OKGM(P < 0.05). In contrast, FABP mRNA expression level in muscle of SPT was significantly decreased by dietary addition of 0.8% OKGM(P < 0.01), yet the amount of OKGM had no significant impact on the expression level of FABP mRNA in liver(P > 0.05). Conclusion: The dietary addition of OKGM can improve the quality of SPT and regulate the gene expression associated with lipid metabolism.
