关键词：
氧化魔芋葡甘露聚糖;C57BL/6J小鼠;十二指肠形态;变性梯度凝胶电泳;肠道菌群
摘 要：
目的:研究氧化魔芋葡甘露聚糖(OKGM)对高脂饮食C57BL/6J小鼠十二指肠形态及肠道菌群的影响,探讨其对高脂饮食小鼠肠道微环境的改善作用。方法:60只雄性C57BL/6J小鼠随机分成基础对照组(CL组,给予基础饲料)、高脂对照组(HF组,给予高脂饲料)及高脂饲料添加低、中、高剂量OKGM(HF-LOKGM组,12.5g/kg;HF-MOKGM组,25.0g/kg;HF-HOKGM组,50.0g/kg)组,共5个实验组。连续饲喂12周后处死小鼠,测定其十二指肠绒毛高度、隐窝深度并通过PCR-DGGE技术探究OKGM对高脂饮食小鼠肠道菌群的影响。结果:CL组十二指肠绒毛高度显著大于其他组(P<0.05),HF组与HF-LOKGM组间无显著差异(P>0.05),与HF-MOKGM组、HF-HOKGM组均有显著差异(P<0.05);HF组十二指肠隐窝深度显著大于其他组(P<0.05),CL组、HF-HOKGM组和HF-MOKGM组3组间差异不显著(P>0.05);各组间十二指肠绒腺比均有显著差异(P<0.05),且呈剂量效应。高脂饮食会改变小鼠的肠道菌群,聚类分析表明HF组与CL组中等不相似,前者多样性指数显著小于后者(P<0.05);添加OKGM组小鼠的肠道菌群更相似于CL组,且HF-HOKGM组多样性指数显著大于HF组(P<0.05),但HF-LOKGM组、HF-MOKGM组与HF组差异均不显著(P>0.05)。结论:OKGM能改善高脂饮食C57BL/6J小鼠肠绒毛形态及肠道菌群。
译 名：
Effect of Oxidized Konjac Glucomannan on Duodenal Morphology and Intestinal Flora in C57BL/6J Mice Fed High-fat Diet
作 者：
WANG Wen-ting;WU Ying-long;College of Food Science,Sichuan Agricultural University;
关键词：
oxidized konjac glucomannan;;C57BL/6J mice;;duodenal morphology;;denaturing gradient gel electrophoresis;;intestinal flora
摘 要：
Objective: To study the effect of oxidized konjac glucomannan(OKGM) on duodenal morphology and intestinal flora in C57BL/6J mice fed high-fat diet. Methods: A total of 60 male C57BL/6J mice were randomly divided into 5 groups: the normal control group(CL, basal diet), high-fat group(HF, high-fat diet) and high-fat diet with low, middle and high dose OKGM(HF-LOKGM, 12.5g/kg; HF-MOKGM, 25.0g/kg; HF-HOKGM, 50.0g/kg) groups. The mice were killed after 12 weeks to determine the duodenal villus height and crypt depth, to explore the effect of OKGM on intestinal flora in C57BL/6J mice fed high-fed diet. Results: The duodenum villus height in the CL group was significantly greater than other groups(P < 0.05), and there was no significant difference between HF group and HF-LOKGM group(P > 0.05), but the HF, HF-MOKGM and HF-HOKGM groups revealed a significant difference(P < 0.05). The duodenum crypt depth in the HF group was significantly larger than other groups(P < 0.05), and a significant difference among the CL, HF-HOKGM and HF-MOKGM groups was observed(P > 0.05). Similarly, a significant difference in the ratio of villus height and crypt depth was also observed(P < 0.05). The intestinal flora in the HF-OKGM group was more similar to the CL group, the diversity index of the HF-HOKGM group was significantly larger than that of the HF group(P < 0.05), but the difference among the HF-LOKGM, HF-MOKGM and HF groups was not significant(P > 0.05). Conclusion: OKGM can improve intestinal villus morphology and intestinal flora in C57BL/6J mice fed high-fat diet.
