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当前位置: 首页 > 文章 > SSR Analysis of Genetic Relationship and Classification in Chrysanthemum Germplasm Collection 园艺学报 2018,4 (2) 73-82
Position: Home > Articles > SSR Analysis of Genetic Relationship and Classification in Chrysanthemum Germplasm Collection Acta Horticulturae Sinica 2018,4 (2) 73-82

SSR Analysis of Genetic Relationship and Classification in Chrysanthemum Germplasm Collection

作  者：
Chao Luo;Dongliang Chen;Xi Cheng;Hua Liu;Yahui Li;Conglin Huan
单  位：
Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center of Functional Floriculture, Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing 100097, China;Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center of Functional Floriculture, Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing 100097, Chin
关键词：
chrysanthemum;SSR;polymorphism;genetic relationship;classification;RAPD ANALYSIS;CULTIVARS;DIVERSITY;MARKERS;DNA;ORIGI
摘  要：
To further test the accuracy and rationality of the Chinese classification system for chrysanthemum (Chrysanthemum morifolium), 10 simple sequence repeat (SSR) markers were used to identify a collection of 88 chrysanthemum and its related genera accessions. In total, 42 effective alleles across 88 accessions were detected; 3 429 bands were obtained by PCR amplification, including 2 630 polymorphic bands. The similarity coefficient ranged from 0.53 to 0.88. Cluster analysis based on UPGMA illustrated that the wild species and large-flower cultivars were first divided into two clusters, then the large-flower cultivars formed five distinct groups according to petal type, indicated that petal type can be a classification criterion. In the wild species cluster, C. vestitum and C. zawadaskii grouped with A. trilobata, suggested that the Ajania genera was closely related to the Chrysanthemum genera. ‘Hangbaiju’, ‘Gongju’ and ‘Chuju’ were grouped together, and ‘Boju’, O. longilobus and C. mongolicum constituted another branch, showed a correlation with geographic region of origin. Population structure analysis was subsequently performed with K values ranging from 2 to 10, and the most likely estimate for the population structure is five subpopulations, which is nearly consistent with the clustering results. Principle component analysis was further performed to verify the classification results. The results showed that these SSR markers are very powerful for studying genetic relationships and will be useful tools in the identification and classification of chrysanthemum.
关键词：
chrysanthemum%SSR%polymorphism%genetic relationship%classification
摘  要：
To further test the accuracy and rationality of the Chinese classification system for chrysanthemum (Chrysanthemum morifolium), 10 simple sequence repeat (SSR) markers were used to identify a collection of 88 chrysanthemum and its related genera accessions. In total, 42 effective alleles across 88 accessions were detected; 3 429 bands were obtained by PCR amplification, including 2 630 polymorphic bands. The similarity coefficient ranged from 0.53 to 0.88. Cluster analysis based on UPGMA illustrated that the wild species and large-flower cultivars were first divided into two clusters, then the large-flower cultivars formed five distinct groups according to petal type, indicated that petal type can be a classification criterion. In the wild species cluster, C. vestitum and C. zawadaskii grouped with A. trilobata, suggested that the Ajania genera was closely related to the Chrysanthemum genera. ‘Hangbaiju’, ‘Gongju’ and ‘Chuju’ were grouped together, and ‘Boju’, O. longilobus and C. mongolicum constituted another branch, showed a correlation with geographic region of origin. Population structure analysis was subsequently performed with K values ranging from 2 to 10, and the most likely estimate for the population structure is five subpopulations, which is nearly consistent with the clustering results. Principle component analysis was further performed to verify the classification results. The results showed that these SSR markers are very powerful for studying genetic relationships and will be useful tools in the identification and classification of chrysanthemum.

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