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Position: Home > Articles > Preparation and Characterization of Monoclonal Antibodies against Aeromonas Sobria Journal of Henan Agricultural Sciences 2006 (2) 108-110

温和气单胞菌单克隆抗体的制备及特性分析

作  者:
黄钧;梁明龙;朱芸
单  位:
广西大学动物科技学院
关键词:
温和气单胞菌;单克隆抗体;表位;酶联免疫吸附分析
摘  要:
利用细胞融合技术建立了5株分泌抗温和气单胞菌CR79-1-1株单克隆抗体(McAb)的杂交瘤细胞株,特异性鉴定获得2株与参考菌株中的温和气单胞菌发生反应,并且与嗜水气单胞菌以及鳗弧菌、爱德华氏菌、克鲁氏耶尔森菌、大肠杆菌、荧光假单胞菌均不反应的杂交瘤细胞,命名为2G3、1A4;快速酶联免疫分析法测得2G3、1A4的亲和常数为5×108L/mol、1.725×108L/mol;应用方阵配对试验证实,2株单抗针对特异性抗原上不同表位。
译  名:
Preparation and Characterization of Monoclonal Antibodies against Aeromonas Sobria
作  者:
HUANG Jun,LIANG Ming-long,ZHU Yun(College of Animal Science and Technology,Guangxi University,Nanning 530004,China)
关键词:
Aeromonas sobria;Monoclonal antibody;Epitope;ELISA
摘  要:
The authors established five hybridoma cell lines against A.sobria strain CR79-1-1 by fusion technique.The specificity analysis by indirect ELISA proved that monoclonal antibodies 2G3 and 1A4 could react with A.sobria.None of them had cross reaction with A.hydrophila,Vibrio anguillarum,Edwardsiella,Yersinia ruckeri,E.coli and P.fluorescent.Their affinity constants were(5×10~8) and 1.725×10~8 L/mol respectively.Different epitopes of A.sobria were recognized by 2G3 and 1A4.

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