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Position: Home > Articles > Sterilization Conditions for Tissue Culture of Stem in Rubia cordifolia L. Journal of Henan Agricultural Sciences 2011,40 (7) 130-131+134

茜草茎段组织培养灭菌方法研究

作  者:
于相丽;李勇慧;王静
单  位:
洛阳师范学院
关键词:
茜草;灭菌方法;污染率;愈伤组织诱导率
摘  要:
研究了不同灭菌剂(0.1%HgCl2、10%NaClO、10%H2O2)、不同灭菌时间、二步灭菌法对茜草不同年龄的茎段污染率和愈伤组织诱导率的影响。结果表明:75%酒精处理30 s+10%NaClO灭菌20 min效果最好,污染率20%,愈伤组织诱导率为35%;二步灭菌法不适合于茜草茎段的灭菌;幼嫩茜草茎段愈伤组织诱导率最高,达55%。
译  名:
Sterilization Conditions for Tissue Culture of Stem in Rubia cordifolia L.
作  者:
YU Xiang-li,LI Yong-hui,WANG Jing(College of Life Science,Luoyang Normal University,Luoyang 471022,China)
关键词:
Rubia cordifolia L.;Sterilization method;Pollution rate;Induction rate
摘  要:
The different age stems from Rubia cordifolia L.were used as explants to study the effects of different bactericides(0.1%HgCl2,10%NaClO,10%H2O2),different sterilization times,and two-step asepsis on pollution of explants and induction of callus.The results showed that the method of 75% alcohol 30s+10% NaClO 20min was the best and the pollution rat was 20%,induction rate was 35%;the method of two-step asepsis was suitable for stems of Rubia cordifolia L.;the young stems'induction rate of callus was the highest up to 55%.

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