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Position: Home > Articles > Primary Studies on Mechanism of Silkworm(Bombyx mori) Resistance to Densovirus China(Zhenjiang) Strain Science of Sericulture 2007,33 (4) 80-85

家蚕对浓核病毒中国(镇江)株抵抗性机制的初步研究

作  者:
裘智勇;李木旺;覃光星;刘挺;沈兴家;郭锡杰
单  位:
中国农业科学院蚕业研究所农业部家蚕生物技术重点开放实验室
关键词:
家蚕;中肠组织;抵抗性;浓核病毒
摘  要:
通过生物测定和地高辛(DIG)标记的酶联免疫吸附剂测定(ELISA),初步分析了家蚕对浓核病毒中国(镇江)株(Bombyxmori densovirus,BmDNV-z)的抵抗性机制。试验表明:供试家蚕品种的消化液、血液中均不存在对BmDNV-z侵染性有影响的蛋白因子;抵抗性家蚕品种的中肠组织蛋白中不存在BmDNV-z的病毒受体蛋白,而感受性家蚕品种的中肠组织蛋白中可能存在对BmDNV-z侵染性有影响的蛋白因子,即感受性家蚕品种的中肠组织中可能存在BmDNV-z的病毒受体蛋白因子,且BmDNV-z与感受性家蚕品种中肠组织蛋白的结合具有组织特异性。
译  名:
Primary Studies on Mechanism of Silkworm(Bombyx mori) Resistance to Densovirus China(Zhenjiang) Strain
作  者:
QIU Zhi-Yong1,2 LI Mu-Wang1 QIN Guang-Xing1 LIU Ting1 SHEN Xing-Jia1 GUO Xi-Jie1,2(1The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Silkworm Biotechnology,Ministry of Agriculture,Zhenjiang Jiangsu 212018,China;2Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212003,China)
关键词:
Bombyx mori;Midgut tissue;Resistance;Densovirus
摘  要:
To investigate the mechanism of silkworm resistance to Bombyx mori densovirus(BmDNV-z),the infectivity of virus to silkworm after incubated with extract of different tissues of silkworm of susceptible and non-susceptible varieties was tested by bio-assay,and the combination activity of BmDNV-z with proteins from the extracts was measured by DIG-labeled ELISA.The results indicated that there were no protein factors affecting the invasion of BmDNV-z in the digestive juice and the blood of the silkworm.Some protein factors which enhanced the invasion of BmDNV-z existed in the midgut of susceptible variety.We speculated that protein factor as virus receptor may exist in the midgut of susceptible variety,but not in the non-susceptible variety.The combination activity was tissue-specific to the midgut tissue of susceptible variety.

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